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Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion

Recent evidence suggests that the beam-induced motion of the sample during tilt-series acquisition is a major resolution-limiting factor in electron cryo-tomography (cryoET). It causes suboptimal tilt-series alignment and thus deterioration of the reconstruction quality. Here we present a novel appr...

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Detalles Bibliográficos
Autores principales: Fernandez, Jose-Jesus, Li, Sam, Bharat, Tanmay A.M., Agard, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5949096/
https://www.ncbi.nlm.nih.gov/pubmed/29410148
http://dx.doi.org/10.1016/j.jsb.2018.02.001
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author Fernandez, Jose-Jesus
Li, Sam
Bharat, Tanmay A.M.
Agard, David A.
author_facet Fernandez, Jose-Jesus
Li, Sam
Bharat, Tanmay A.M.
Agard, David A.
author_sort Fernandez, Jose-Jesus
collection PubMed
description Recent evidence suggests that the beam-induced motion of the sample during tilt-series acquisition is a major resolution-limiting factor in electron cryo-tomography (cryoET). It causes suboptimal tilt-series alignment and thus deterioration of the reconstruction quality. Here we present a novel approach to tilt-series alignment and tomographic reconstruction that considers the beam-induced sample motion through the tilt-series. It extends the standard fiducial-based alignment approach in cryoET by introducing quadratic polynomials to model the sample motion. The model can be used during reconstruction to yield a motion-compensated tomogram. We evaluated our method on various datasets with different sample sizes. The results demonstrate that our method could be a useful tool to improve the quality of tomograms and the resolution in cryoET.
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spelling pubmed-59490962018-06-01 Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion Fernandez, Jose-Jesus Li, Sam Bharat, Tanmay A.M. Agard, David A. J Struct Biol Article Recent evidence suggests that the beam-induced motion of the sample during tilt-series acquisition is a major resolution-limiting factor in electron cryo-tomography (cryoET). It causes suboptimal tilt-series alignment and thus deterioration of the reconstruction quality. Here we present a novel approach to tilt-series alignment and tomographic reconstruction that considers the beam-induced sample motion through the tilt-series. It extends the standard fiducial-based alignment approach in cryoET by introducing quadratic polynomials to model the sample motion. The model can be used during reconstruction to yield a motion-compensated tomogram. We evaluated our method on various datasets with different sample sizes. The results demonstrate that our method could be a useful tool to improve the quality of tomograms and the resolution in cryoET. Academic Press 2018-06 /pmc/articles/PMC5949096/ /pubmed/29410148 http://dx.doi.org/10.1016/j.jsb.2018.02.001 Text en © 2018 MRC Laboratory of Molecular Biology http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fernandez, Jose-Jesus
Li, Sam
Bharat, Tanmay A.M.
Agard, David A.
Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion
title Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion
title_full Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion
title_fullStr Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion
title_full_unstemmed Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion
title_short Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion
title_sort cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5949096/
https://www.ncbi.nlm.nih.gov/pubmed/29410148
http://dx.doi.org/10.1016/j.jsb.2018.02.001
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