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FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase-mediated pathway

In the present study, the triterpenoid 3-acetoxylanosta-8,24-dien-21-oic acid (FPOA) was extracted from Fomitopsis pinicola. The aim of the present was to elucidate the mechanism of action of FPOA in HeLa cervical cancer cells. Cell viability was examined using an MTT assay and the morphological det...

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Detalles Bibliográficos
Autores principales: Li, Xin, Bau, Tolgor, Bao, Haiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5950026/
https://www.ncbi.nlm.nih.gov/pubmed/29805569
http://dx.doi.org/10.3892/ol.2018.8380
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author Li, Xin
Bau, Tolgor
Bao, Haiying
author_facet Li, Xin
Bau, Tolgor
Bao, Haiying
author_sort Li, Xin
collection PubMed
description In the present study, the triterpenoid 3-acetoxylanosta-8,24-dien-21-oic acid (FPOA) was extracted from Fomitopsis pinicola. The aim of the present was to elucidate the mechanism of action of FPOA in HeLa cervical cancer cells. Cell viability was examined using an MTT assay and the morphological detection of apoptosis was conducted using DAPI staining. The rate of apoptosis was examined via Annexin V-FITC/PI double staining and the expression levels of apoptosis-associated proteins were determined by western blot analysis. FPOA was observed to inhibit HeLa cell proliferation, with IC(50) values of 25.28, 15.30 and 11.79 µg/ml at 24, 48 and 72 h, respectively. Typical apoptotic bodies were observed in the HeLa cells following treatment with FPOA, as revealed by DAPI staining. The percentage of apoptotic cells was 3.00, 3.12, 6.18 and 32.28% following treatment with FPOA at concentrations of 0, 7.5, 15 and 30 µg/ml, respectively. Western blot analysis showed that caspase-3 and −9 were cleaved more frequently after treatment with FPOA. Furthermore, the expression of Bax was increased but Bcl-2 expression was decreased after treatment with FPOA. These results suggest that FPOA can induce HeLa cell apoptosis through a caspase-mediated pathway.
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spelling pubmed-59500262018-05-27 FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase-mediated pathway Li, Xin Bau, Tolgor Bao, Haiying Oncol Lett Articles In the present study, the triterpenoid 3-acetoxylanosta-8,24-dien-21-oic acid (FPOA) was extracted from Fomitopsis pinicola. The aim of the present was to elucidate the mechanism of action of FPOA in HeLa cervical cancer cells. Cell viability was examined using an MTT assay and the morphological detection of apoptosis was conducted using DAPI staining. The rate of apoptosis was examined via Annexin V-FITC/PI double staining and the expression levels of apoptosis-associated proteins were determined by western blot analysis. FPOA was observed to inhibit HeLa cell proliferation, with IC(50) values of 25.28, 15.30 and 11.79 µg/ml at 24, 48 and 72 h, respectively. Typical apoptotic bodies were observed in the HeLa cells following treatment with FPOA, as revealed by DAPI staining. The percentage of apoptotic cells was 3.00, 3.12, 6.18 and 32.28% following treatment with FPOA at concentrations of 0, 7.5, 15 and 30 µg/ml, respectively. Western blot analysis showed that caspase-3 and −9 were cleaved more frequently after treatment with FPOA. Furthermore, the expression of Bax was increased but Bcl-2 expression was decreased after treatment with FPOA. These results suggest that FPOA can induce HeLa cell apoptosis through a caspase-mediated pathway. D.A. Spandidos 2018-06 2018-03-29 /pmc/articles/PMC5950026/ /pubmed/29805569 http://dx.doi.org/10.3892/ol.2018.8380 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Xin
Bau, Tolgor
Bao, Haiying
FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase-mediated pathway
title FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase-mediated pathway
title_full FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase-mediated pathway
title_fullStr FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase-mediated pathway
title_full_unstemmed FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase-mediated pathway
title_short FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase-mediated pathway
title_sort fpoa induces apoptosis in hela human cervical cancer cells through a caspase-mediated pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5950026/
https://www.ncbi.nlm.nih.gov/pubmed/29805569
http://dx.doi.org/10.3892/ol.2018.8380
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