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miR-100 inhibits the migration and invasion of nasopharyngeal carcinoma by targeting IGF1R

Nasopharyngeal carcinoma (NPC) is a cancer pattern that often develops in the epithelial cells of the nasopharynx. miR-100 is a miRNA that has been identified in a number of cancers. The aim of the present study was to investigate whether miR-100 can affect cell migration and proliferation of NPC by...

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Autores principales: Sun, Xiaoyan, Liu, Xiaoying, Wang, Yanmei, Yang, Shuqin, Chen, Yao, Yuan, Tiejun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5950178/
https://www.ncbi.nlm.nih.gov/pubmed/29805566
http://dx.doi.org/10.3892/ol.2018.8420
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author Sun, Xiaoyan
Liu, Xiaoying
Wang, Yanmei
Yang, Shuqin
Chen, Yao
Yuan, Tiejun
author_facet Sun, Xiaoyan
Liu, Xiaoying
Wang, Yanmei
Yang, Shuqin
Chen, Yao
Yuan, Tiejun
author_sort Sun, Xiaoyan
collection PubMed
description Nasopharyngeal carcinoma (NPC) is a cancer pattern that often develops in the epithelial cells of the nasopharynx. miR-100 is a miRNA that has been identified in a number of cancers. The aim of the present study was to investigate whether miR-100 can affect cell migration and proliferation of NPC by targeting insulin-like growth factor 1 receptor (IGF1R). Western blot analysis was used to determine the protein levels of genes. The reverse transcription-quantitative PCR (RT-qPCR) was used to detect the expression level of miR-100 and IGF1R. Transwell assay was used to detect the migration and invasion of cell lines. The luciferase reporter assay was employed to confirm the target gene of miR-100. miR-100 expression was highly reduced in NPC tissues compared with non-cancerous tissues. Overexpression of miR-100 significantly inhibited the migration and invasion of NPC cell lines C666-1 and SUNE1. IGF1R was a downstream target of miR-100 and was downregulated by miR-100. Knockdown of IGF1R by siRNA suppressed cell proliferation of the C666-1 cell line. The newly identified miR-100/IGF1R axis offers novel biomarkers for the therapeutic intervention of NPC treatment. As a result, our findings suggest that miR-100 plays an important role in suppressing migration and invasion of NPC cells and suppresses IGF1R expression by directly targeting its 3′-UTR. It is suggested that miR-100 may be a novel therapeutic target of microRNA-mediated suppression of cell migration and invasion in NPC. However, the role of the miR-100/IGF1R axis in NPC progression needs further investigation.
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spelling pubmed-59501782018-05-27 miR-100 inhibits the migration and invasion of nasopharyngeal carcinoma by targeting IGF1R Sun, Xiaoyan Liu, Xiaoying Wang, Yanmei Yang, Shuqin Chen, Yao Yuan, Tiejun Oncol Lett Articles Nasopharyngeal carcinoma (NPC) is a cancer pattern that often develops in the epithelial cells of the nasopharynx. miR-100 is a miRNA that has been identified in a number of cancers. The aim of the present study was to investigate whether miR-100 can affect cell migration and proliferation of NPC by targeting insulin-like growth factor 1 receptor (IGF1R). Western blot analysis was used to determine the protein levels of genes. The reverse transcription-quantitative PCR (RT-qPCR) was used to detect the expression level of miR-100 and IGF1R. Transwell assay was used to detect the migration and invasion of cell lines. The luciferase reporter assay was employed to confirm the target gene of miR-100. miR-100 expression was highly reduced in NPC tissues compared with non-cancerous tissues. Overexpression of miR-100 significantly inhibited the migration and invasion of NPC cell lines C666-1 and SUNE1. IGF1R was a downstream target of miR-100 and was downregulated by miR-100. Knockdown of IGF1R by siRNA suppressed cell proliferation of the C666-1 cell line. The newly identified miR-100/IGF1R axis offers novel biomarkers for the therapeutic intervention of NPC treatment. As a result, our findings suggest that miR-100 plays an important role in suppressing migration and invasion of NPC cells and suppresses IGF1R expression by directly targeting its 3′-UTR. It is suggested that miR-100 may be a novel therapeutic target of microRNA-mediated suppression of cell migration and invasion in NPC. However, the role of the miR-100/IGF1R axis in NPC progression needs further investigation. D.A. Spandidos 2018-06 2018-04-04 /pmc/articles/PMC5950178/ /pubmed/29805566 http://dx.doi.org/10.3892/ol.2018.8420 Text en Copyright: © Sun et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Sun, Xiaoyan
Liu, Xiaoying
Wang, Yanmei
Yang, Shuqin
Chen, Yao
Yuan, Tiejun
miR-100 inhibits the migration and invasion of nasopharyngeal carcinoma by targeting IGF1R
title miR-100 inhibits the migration and invasion of nasopharyngeal carcinoma by targeting IGF1R
title_full miR-100 inhibits the migration and invasion of nasopharyngeal carcinoma by targeting IGF1R
title_fullStr miR-100 inhibits the migration and invasion of nasopharyngeal carcinoma by targeting IGF1R
title_full_unstemmed miR-100 inhibits the migration and invasion of nasopharyngeal carcinoma by targeting IGF1R
title_short miR-100 inhibits the migration and invasion of nasopharyngeal carcinoma by targeting IGF1R
title_sort mir-100 inhibits the migration and invasion of nasopharyngeal carcinoma by targeting igf1r
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5950178/
https://www.ncbi.nlm.nih.gov/pubmed/29805566
http://dx.doi.org/10.3892/ol.2018.8420
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