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Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor

BACKGROUND: Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packed-bed biofilm reactor (PBBR) was investigated. RESULTS: The effects of...

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Detalles Bibliográficos
Autores principales: Ferone, Mariateresa, Raganati, Francesca, Ercole, Alessia, Olivieri, Giuseppe, Salatino, Piero, Marzocchella, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5950251/
https://www.ncbi.nlm.nih.gov/pubmed/29785205
http://dx.doi.org/10.1186/s13068-018-1143-7
Descripción
Sumario:BACKGROUND: Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packed-bed biofilm reactor (PBBR) was investigated. RESULTS: The effects of the operating conditions tested, dilution rate (D), and medium composition (mixture of glucose, xylose, and arabinose—that simulate the composition of a lignocellulosic hydrolysate)—on the PBBR performances were investigated. The maximum succinic acid productivity of 35.0 g L(−1) h(−1) and the maximum SA concentration were achieved at a D = 1.9 h(−1). The effect of HMF and furfural on succinic acid production was also investigated. HMF resulted to reduce succinic acid production by 22.6%, while furfural caused a reduction of 16% in SA production at the same dilution rate. CONCLUSION: Succinic acid production by A. succinogenes fermentation in a packed-bed reactor (PBBR) was successfully carried out for more than 5 months. The optimal results were obtained at the dilution rate 0.5 h(−1): 43.0 g L(−1) of succinic acid were produced, glucose conversion was 88%; and the volumetric productivity was 22 g L(−1) h(−1). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-018-1143-7) contains supplementary material, which is available to authorized users.