Cargando…
Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor
BACKGROUND: Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packed-bed biofilm reactor (PBBR) was investigated. RESULTS: The effects of...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5950251/ https://www.ncbi.nlm.nih.gov/pubmed/29785205 http://dx.doi.org/10.1186/s13068-018-1143-7 |
_version_ | 1783322852770971648 |
---|---|
author | Ferone, Mariateresa Raganati, Francesca Ercole, Alessia Olivieri, Giuseppe Salatino, Piero Marzocchella, Antonio |
author_facet | Ferone, Mariateresa Raganati, Francesca Ercole, Alessia Olivieri, Giuseppe Salatino, Piero Marzocchella, Antonio |
author_sort | Ferone, Mariateresa |
collection | PubMed |
description | BACKGROUND: Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packed-bed biofilm reactor (PBBR) was investigated. RESULTS: The effects of the operating conditions tested, dilution rate (D), and medium composition (mixture of glucose, xylose, and arabinose—that simulate the composition of a lignocellulosic hydrolysate)—on the PBBR performances were investigated. The maximum succinic acid productivity of 35.0 g L(−1) h(−1) and the maximum SA concentration were achieved at a D = 1.9 h(−1). The effect of HMF and furfural on succinic acid production was also investigated. HMF resulted to reduce succinic acid production by 22.6%, while furfural caused a reduction of 16% in SA production at the same dilution rate. CONCLUSION: Succinic acid production by A. succinogenes fermentation in a packed-bed reactor (PBBR) was successfully carried out for more than 5 months. The optimal results were obtained at the dilution rate 0.5 h(−1): 43.0 g L(−1) of succinic acid were produced, glucose conversion was 88%; and the volumetric productivity was 22 g L(−1) h(−1). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-018-1143-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5950251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-59502512018-05-21 Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor Ferone, Mariateresa Raganati, Francesca Ercole, Alessia Olivieri, Giuseppe Salatino, Piero Marzocchella, Antonio Biotechnol Biofuels Research BACKGROUND: Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packed-bed biofilm reactor (PBBR) was investigated. RESULTS: The effects of the operating conditions tested, dilution rate (D), and medium composition (mixture of glucose, xylose, and arabinose—that simulate the composition of a lignocellulosic hydrolysate)—on the PBBR performances were investigated. The maximum succinic acid productivity of 35.0 g L(−1) h(−1) and the maximum SA concentration were achieved at a D = 1.9 h(−1). The effect of HMF and furfural on succinic acid production was also investigated. HMF resulted to reduce succinic acid production by 22.6%, while furfural caused a reduction of 16% in SA production at the same dilution rate. CONCLUSION: Succinic acid production by A. succinogenes fermentation in a packed-bed reactor (PBBR) was successfully carried out for more than 5 months. The optimal results were obtained at the dilution rate 0.5 h(−1): 43.0 g L(−1) of succinic acid were produced, glucose conversion was 88%; and the volumetric productivity was 22 g L(−1) h(−1). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-018-1143-7) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-14 /pmc/articles/PMC5950251/ /pubmed/29785205 http://dx.doi.org/10.1186/s13068-018-1143-7 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Ferone, Mariateresa Raganati, Francesca Ercole, Alessia Olivieri, Giuseppe Salatino, Piero Marzocchella, Antonio Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor |
title | Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor |
title_full | Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor |
title_fullStr | Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor |
title_full_unstemmed | Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor |
title_short | Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor |
title_sort | continuous succinic acid fermentation by actinobacillus succinogenes in a packed-bed biofilm reactor |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5950251/ https://www.ncbi.nlm.nih.gov/pubmed/29785205 http://dx.doi.org/10.1186/s13068-018-1143-7 |
work_keys_str_mv | AT feronemariateresa continuoussuccinicacidfermentationbyactinobacillussuccinogenesinapackedbedbiofilmreactor AT raganatifrancesca continuoussuccinicacidfermentationbyactinobacillussuccinogenesinapackedbedbiofilmreactor AT ercolealessia continuoussuccinicacidfermentationbyactinobacillussuccinogenesinapackedbedbiofilmreactor AT olivierigiuseppe continuoussuccinicacidfermentationbyactinobacillussuccinogenesinapackedbedbiofilmreactor AT salatinopiero continuoussuccinicacidfermentationbyactinobacillussuccinogenesinapackedbedbiofilmreactor AT marzocchellaantonio continuoussuccinicacidfermentationbyactinobacillussuccinogenesinapackedbedbiofilmreactor |