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Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor

BACKGROUND: Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packed-bed biofilm reactor (PBBR) was investigated. RESULTS: The effects of...

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Autores principales: Ferone, Mariateresa, Raganati, Francesca, Ercole, Alessia, Olivieri, Giuseppe, Salatino, Piero, Marzocchella, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5950251/
https://www.ncbi.nlm.nih.gov/pubmed/29785205
http://dx.doi.org/10.1186/s13068-018-1143-7
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author Ferone, Mariateresa
Raganati, Francesca
Ercole, Alessia
Olivieri, Giuseppe
Salatino, Piero
Marzocchella, Antonio
author_facet Ferone, Mariateresa
Raganati, Francesca
Ercole, Alessia
Olivieri, Giuseppe
Salatino, Piero
Marzocchella, Antonio
author_sort Ferone, Mariateresa
collection PubMed
description BACKGROUND: Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packed-bed biofilm reactor (PBBR) was investigated. RESULTS: The effects of the operating conditions tested, dilution rate (D), and medium composition (mixture of glucose, xylose, and arabinose—that simulate the composition of a lignocellulosic hydrolysate)—on the PBBR performances were investigated. The maximum succinic acid productivity of 35.0 g L(−1) h(−1) and the maximum SA concentration were achieved at a D = 1.9 h(−1). The effect of HMF and furfural on succinic acid production was also investigated. HMF resulted to reduce succinic acid production by 22.6%, while furfural caused a reduction of 16% in SA production at the same dilution rate. CONCLUSION: Succinic acid production by A. succinogenes fermentation in a packed-bed reactor (PBBR) was successfully carried out for more than 5 months. The optimal results were obtained at the dilution rate 0.5 h(−1): 43.0 g L(−1) of succinic acid were produced, glucose conversion was 88%; and the volumetric productivity was 22 g L(−1) h(−1). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-018-1143-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-59502512018-05-21 Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor Ferone, Mariateresa Raganati, Francesca Ercole, Alessia Olivieri, Giuseppe Salatino, Piero Marzocchella, Antonio Biotechnol Biofuels Research BACKGROUND: Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packed-bed biofilm reactor (PBBR) was investigated. RESULTS: The effects of the operating conditions tested, dilution rate (D), and medium composition (mixture of glucose, xylose, and arabinose—that simulate the composition of a lignocellulosic hydrolysate)—on the PBBR performances were investigated. The maximum succinic acid productivity of 35.0 g L(−1) h(−1) and the maximum SA concentration were achieved at a D = 1.9 h(−1). The effect of HMF and furfural on succinic acid production was also investigated. HMF resulted to reduce succinic acid production by 22.6%, while furfural caused a reduction of 16% in SA production at the same dilution rate. CONCLUSION: Succinic acid production by A. succinogenes fermentation in a packed-bed reactor (PBBR) was successfully carried out for more than 5 months. The optimal results were obtained at the dilution rate 0.5 h(−1): 43.0 g L(−1) of succinic acid were produced, glucose conversion was 88%; and the volumetric productivity was 22 g L(−1) h(−1). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-018-1143-7) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-14 /pmc/articles/PMC5950251/ /pubmed/29785205 http://dx.doi.org/10.1186/s13068-018-1143-7 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ferone, Mariateresa
Raganati, Francesca
Ercole, Alessia
Olivieri, Giuseppe
Salatino, Piero
Marzocchella, Antonio
Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor
title Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor
title_full Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor
title_fullStr Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor
title_full_unstemmed Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor
title_short Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor
title_sort continuous succinic acid fermentation by actinobacillus succinogenes in a packed-bed biofilm reactor
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5950251/
https://www.ncbi.nlm.nih.gov/pubmed/29785205
http://dx.doi.org/10.1186/s13068-018-1143-7
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