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Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces

Titanium is a biocompatible material that is frequently used for making implantable medical devices. Nanoengineering of the surface is the common method for increasing material biocompatibility, and while the nanostructured materials are well-known to represent attractive substrata for eukaryotic ce...

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Autores principales: Wandiyanto, Jason V., Linklater, Denver, Tharushi Perera, Pallale G., Orlowska, Anna, Truong, Vi Khanh, Thissen, Helmut, Ghanaati, Shahram, Baulin, Vladimir, Crawford, Russell J., Juodkazis, Saulius, Ivanova, Elena P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5951489/
https://www.ncbi.nlm.nih.gov/pubmed/29662020
http://dx.doi.org/10.3390/ma11040605
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author Wandiyanto, Jason V.
Linklater, Denver
Tharushi Perera, Pallale G.
Orlowska, Anna
Truong, Vi Khanh
Thissen, Helmut
Ghanaati, Shahram
Baulin, Vladimir
Crawford, Russell J.
Juodkazis, Saulius
Ivanova, Elena P.
author_facet Wandiyanto, Jason V.
Linklater, Denver
Tharushi Perera, Pallale G.
Orlowska, Anna
Truong, Vi Khanh
Thissen, Helmut
Ghanaati, Shahram
Baulin, Vladimir
Crawford, Russell J.
Juodkazis, Saulius
Ivanova, Elena P.
author_sort Wandiyanto, Jason V.
collection PubMed
description Titanium is a biocompatible material that is frequently used for making implantable medical devices. Nanoengineering of the surface is the common method for increasing material biocompatibility, and while the nanostructured materials are well-known to represent attractive substrata for eukaryotic cells, very little information has been documented about the interaction between mammalian cells and bactericidal nanostructured surfaces. In this study, we investigated the effect of bactericidal titanium nanostructures on PC12 cell attachment and differentiation—a cell line which has become a widely used in vitro model to study neuronal differentiation. The effects of the nanostructures on the cells were then compared to effects observed when the cells were placed in contact with non-structured titanium. It was found that bactericidal nanostructured surfaces enhanced the attachment of neuron-like cells. In addition, the PC12 cells were able to differentiate on nanostructured surfaces, while the cells on non-structured surfaces were not able to do so. These promising results demonstrate the potential application of bactericidal nanostructured surfaces in biomedical applications such as cochlear and neuronal implants.
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spelling pubmed-59514892018-05-15 Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces Wandiyanto, Jason V. Linklater, Denver Tharushi Perera, Pallale G. Orlowska, Anna Truong, Vi Khanh Thissen, Helmut Ghanaati, Shahram Baulin, Vladimir Crawford, Russell J. Juodkazis, Saulius Ivanova, Elena P. Materials (Basel) Article Titanium is a biocompatible material that is frequently used for making implantable medical devices. Nanoengineering of the surface is the common method for increasing material biocompatibility, and while the nanostructured materials are well-known to represent attractive substrata for eukaryotic cells, very little information has been documented about the interaction between mammalian cells and bactericidal nanostructured surfaces. In this study, we investigated the effect of bactericidal titanium nanostructures on PC12 cell attachment and differentiation—a cell line which has become a widely used in vitro model to study neuronal differentiation. The effects of the nanostructures on the cells were then compared to effects observed when the cells were placed in contact with non-structured titanium. It was found that bactericidal nanostructured surfaces enhanced the attachment of neuron-like cells. In addition, the PC12 cells were able to differentiate on nanostructured surfaces, while the cells on non-structured surfaces were not able to do so. These promising results demonstrate the potential application of bactericidal nanostructured surfaces in biomedical applications such as cochlear and neuronal implants. MDPI 2018-04-14 /pmc/articles/PMC5951489/ /pubmed/29662020 http://dx.doi.org/10.3390/ma11040605 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wandiyanto, Jason V.
Linklater, Denver
Tharushi Perera, Pallale G.
Orlowska, Anna
Truong, Vi Khanh
Thissen, Helmut
Ghanaati, Shahram
Baulin, Vladimir
Crawford, Russell J.
Juodkazis, Saulius
Ivanova, Elena P.
Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces
title Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces
title_full Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces
title_fullStr Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces
title_full_unstemmed Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces
title_short Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces
title_sort pheochromocytoma (pc12) cell response on mechanobactericidal titanium surfaces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5951489/
https://www.ncbi.nlm.nih.gov/pubmed/29662020
http://dx.doi.org/10.3390/ma11040605
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