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Quantifying compressive forces between living cell layers and within tissues using elastic round microgels

Increasing evidence shows that mechanical stresses are critical in regulating cell functions, fate, and diseases. However, no methods exist that can quantify isotropic compressive stresses. Here we describe fluorescent nanoparticle-labeled, monodisperse elastic microspheres made of Arg-Gly-Asp-conju...

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Autores principales: Mohagheghian, Erfan, Luo, Junyu, Chen, Junjian, Chaudhary, Gaurav, Chen, Junwei, Sun, Jian, Ewoldt, Randy H., Wang, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5951850/
https://www.ncbi.nlm.nih.gov/pubmed/29760452
http://dx.doi.org/10.1038/s41467-018-04245-1
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author Mohagheghian, Erfan
Luo, Junyu
Chen, Junjian
Chaudhary, Gaurav
Chen, Junwei
Sun, Jian
Ewoldt, Randy H.
Wang, Ning
author_facet Mohagheghian, Erfan
Luo, Junyu
Chen, Junjian
Chaudhary, Gaurav
Chen, Junwei
Sun, Jian
Ewoldt, Randy H.
Wang, Ning
author_sort Mohagheghian, Erfan
collection PubMed
description Increasing evidence shows that mechanical stresses are critical in regulating cell functions, fate, and diseases. However, no methods exist that can quantify isotropic compressive stresses. Here we describe fluorescent nanoparticle-labeled, monodisperse elastic microspheres made of Arg-Gly-Asp-conjugated alginate hydrogels (elastic round microgels, ERMGs). We generate 3D displacements and calculate strains and tractions exerted on an ERMG. Average compressive tractions on an ERMG are 570 Pa within cell layers and 360 Pa in tumor-repopulating cell (TRC) colonies grown in 400-Pa matrices. 3D compressive tractions on a 1.4-kPa ERMG are applied by surrounding cells via endogenous actomyosin forces but not via mature focal adhesions. Compressive stresses are substantially heterogeneous on ERMGs within a uniform cell colony and do not increase with TRC colony sizes. Early-stage zebrafish embryos generate spatial and temporal differences in local normal and shear stresses. This ERMG method could be useful for quantifying stresses in vitro and in vivo.
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spelling pubmed-59518502018-05-16 Quantifying compressive forces between living cell layers and within tissues using elastic round microgels Mohagheghian, Erfan Luo, Junyu Chen, Junjian Chaudhary, Gaurav Chen, Junwei Sun, Jian Ewoldt, Randy H. Wang, Ning Nat Commun Article Increasing evidence shows that mechanical stresses are critical in regulating cell functions, fate, and diseases. However, no methods exist that can quantify isotropic compressive stresses. Here we describe fluorescent nanoparticle-labeled, monodisperse elastic microspheres made of Arg-Gly-Asp-conjugated alginate hydrogels (elastic round microgels, ERMGs). We generate 3D displacements and calculate strains and tractions exerted on an ERMG. Average compressive tractions on an ERMG are 570 Pa within cell layers and 360 Pa in tumor-repopulating cell (TRC) colonies grown in 400-Pa matrices. 3D compressive tractions on a 1.4-kPa ERMG are applied by surrounding cells via endogenous actomyosin forces but not via mature focal adhesions. Compressive stresses are substantially heterogeneous on ERMGs within a uniform cell colony and do not increase with TRC colony sizes. Early-stage zebrafish embryos generate spatial and temporal differences in local normal and shear stresses. This ERMG method could be useful for quantifying stresses in vitro and in vivo. Nature Publishing Group UK 2018-05-14 /pmc/articles/PMC5951850/ /pubmed/29760452 http://dx.doi.org/10.1038/s41467-018-04245-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mohagheghian, Erfan
Luo, Junyu
Chen, Junjian
Chaudhary, Gaurav
Chen, Junwei
Sun, Jian
Ewoldt, Randy H.
Wang, Ning
Quantifying compressive forces between living cell layers and within tissues using elastic round microgels
title Quantifying compressive forces between living cell layers and within tissues using elastic round microgels
title_full Quantifying compressive forces between living cell layers and within tissues using elastic round microgels
title_fullStr Quantifying compressive forces between living cell layers and within tissues using elastic round microgels
title_full_unstemmed Quantifying compressive forces between living cell layers and within tissues using elastic round microgels
title_short Quantifying compressive forces between living cell layers and within tissues using elastic round microgels
title_sort quantifying compressive forces between living cell layers and within tissues using elastic round microgels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5951850/
https://www.ncbi.nlm.nih.gov/pubmed/29760452
http://dx.doi.org/10.1038/s41467-018-04245-1
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