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Genomic evidence for intraspecific hybridization in a clonal and extremely halotolerant yeast
BACKGROUND: The black yeast Hortaea werneckii (Dothideomycetes, Ascomycota) is one of the most extremely halotolerant fungi, capable of growth at NaCl concentrations close to saturation. Although dothideomycetous fungi are typically haploid, the reference H. werneckii strain has a diploid genome con...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5952469/ https://www.ncbi.nlm.nih.gov/pubmed/29764372 http://dx.doi.org/10.1186/s12864-018-4751-5 |
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author | Gostinčar, Cene Stajich, Jason E. Zupančič, Jerneja Zalar, Polona Gunde-Cimerman, Nina |
author_facet | Gostinčar, Cene Stajich, Jason E. Zupančič, Jerneja Zalar, Polona Gunde-Cimerman, Nina |
author_sort | Gostinčar, Cene |
collection | PubMed |
description | BACKGROUND: The black yeast Hortaea werneckii (Dothideomycetes, Ascomycota) is one of the most extremely halotolerant fungi, capable of growth at NaCl concentrations close to saturation. Although dothideomycetous fungi are typically haploid, the reference H. werneckii strain has a diploid genome consisting of two subgenomes with a high level of heterozygosity. RESULTS: In order to explain the origin of the H. werneckii diploid genome we here report the genome sequencing of eleven strains isolated from different habitats and geographic locations. Comparison of nine diploid and two haploid strains showed that the reference genome was likely formed by hybridization between two haploids and not by endoreduplication as suggested previously. Results also support additional hybridization events in the evolutionary history of investigated strains, however exchange of genetic material in the species otherwise appears to be rare. Possible links between such unusual reproduction and the extremotolerance of H. werneckii remain to be investigated. CONCLUSIONS: H. werneckii appears to be able to form persistent haploid as well as diploid strains, is capable of occasional hybridization between relatively heterozygous haploids, but is otherwise limited to clonal reproduction. The reported data and the first identification of haploid H. werneckii strains establish this species as a good model for studying the effects of ploidy and hybridization in an extremotolerant system unperturbed by frequent genetic recombination. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4751-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5952469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-59524692018-05-21 Genomic evidence for intraspecific hybridization in a clonal and extremely halotolerant yeast Gostinčar, Cene Stajich, Jason E. Zupančič, Jerneja Zalar, Polona Gunde-Cimerman, Nina BMC Genomics Research Article BACKGROUND: The black yeast Hortaea werneckii (Dothideomycetes, Ascomycota) is one of the most extremely halotolerant fungi, capable of growth at NaCl concentrations close to saturation. Although dothideomycetous fungi are typically haploid, the reference H. werneckii strain has a diploid genome consisting of two subgenomes with a high level of heterozygosity. RESULTS: In order to explain the origin of the H. werneckii diploid genome we here report the genome sequencing of eleven strains isolated from different habitats and geographic locations. Comparison of nine diploid and two haploid strains showed that the reference genome was likely formed by hybridization between two haploids and not by endoreduplication as suggested previously. Results also support additional hybridization events in the evolutionary history of investigated strains, however exchange of genetic material in the species otherwise appears to be rare. Possible links between such unusual reproduction and the extremotolerance of H. werneckii remain to be investigated. CONCLUSIONS: H. werneckii appears to be able to form persistent haploid as well as diploid strains, is capable of occasional hybridization between relatively heterozygous haploids, but is otherwise limited to clonal reproduction. The reported data and the first identification of haploid H. werneckii strains establish this species as a good model for studying the effects of ploidy and hybridization in an extremotolerant system unperturbed by frequent genetic recombination. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4751-5) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-15 /pmc/articles/PMC5952469/ /pubmed/29764372 http://dx.doi.org/10.1186/s12864-018-4751-5 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Gostinčar, Cene Stajich, Jason E. Zupančič, Jerneja Zalar, Polona Gunde-Cimerman, Nina Genomic evidence for intraspecific hybridization in a clonal and extremely halotolerant yeast |
title | Genomic evidence for intraspecific hybridization in a clonal and extremely halotolerant yeast |
title_full | Genomic evidence for intraspecific hybridization in a clonal and extremely halotolerant yeast |
title_fullStr | Genomic evidence for intraspecific hybridization in a clonal and extremely halotolerant yeast |
title_full_unstemmed | Genomic evidence for intraspecific hybridization in a clonal and extremely halotolerant yeast |
title_short | Genomic evidence for intraspecific hybridization in a clonal and extremely halotolerant yeast |
title_sort | genomic evidence for intraspecific hybridization in a clonal and extremely halotolerant yeast |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5952469/ https://www.ncbi.nlm.nih.gov/pubmed/29764372 http://dx.doi.org/10.1186/s12864-018-4751-5 |
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