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Antibacterial Activity of Two Flavans from the Stem Bark of Embelia schimperi

Embelia schemperi Vatke is one of the medicinal plants used traditionally for treatment of intestinal tape worm, dysmenorrheal, bacterial, and fungal infections. Phytochemical screening test of the dichloromethane/methanol (1 : 1) and methanol extracts revealed the presence of phenols, alkaloids, ta...

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Autores principales: Guyasa, Babe, Melaku, Yadessa, Endale, Milkyas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5952501/
https://www.ncbi.nlm.nih.gov/pubmed/29853867
http://dx.doi.org/10.1155/2018/5870161
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author Guyasa, Babe
Melaku, Yadessa
Endale, Milkyas
author_facet Guyasa, Babe
Melaku, Yadessa
Endale, Milkyas
author_sort Guyasa, Babe
collection PubMed
description Embelia schemperi Vatke is one of the medicinal plants used traditionally for treatment of intestinal tape worm, dysmenorrheal, bacterial, and fungal infections. Phytochemical screening test of the dichloromethane/methanol (1 : 1) and methanol extracts revealed the presence of phenols, alkaloids, tannins, and flavonoids whereas terpenoids, glycoside, and phytosterols were absent. Silica gel column chromatographic separation of the methanol extract afforded 3,5,7,3′,4′-pentahydroxyflavan, named epicatechin (1), along with a close flavan derivative (2). Structures of the compounds were elucidated by spectroscopic techniques (1D and 2D NMR, FTIR, and UV-Vis). The crude extracts and isolated compounds were screened for in vitro antibacterial activity against strains of Klebsiella pneumoniae, Escherichia coli, Cryptococcus neoformans, Shigella dysentriae, and Staphylococcus aureus. Epicatechin (1) exhibited comparable antibacterial activity against S. aureus and E. coli (15 and 12 mm zone of inhibition, resp.) to that of the control antibiotic gentamicin, with zone of inhibition of 15 and 12 mm, respectively, at a concentration of 20 µg/mL.
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spelling pubmed-59525012018-05-31 Antibacterial Activity of Two Flavans from the Stem Bark of Embelia schimperi Guyasa, Babe Melaku, Yadessa Endale, Milkyas Adv Pharmacol Sci Research Article Embelia schemperi Vatke is one of the medicinal plants used traditionally for treatment of intestinal tape worm, dysmenorrheal, bacterial, and fungal infections. Phytochemical screening test of the dichloromethane/methanol (1 : 1) and methanol extracts revealed the presence of phenols, alkaloids, tannins, and flavonoids whereas terpenoids, glycoside, and phytosterols were absent. Silica gel column chromatographic separation of the methanol extract afforded 3,5,7,3′,4′-pentahydroxyflavan, named epicatechin (1), along with a close flavan derivative (2). Structures of the compounds were elucidated by spectroscopic techniques (1D and 2D NMR, FTIR, and UV-Vis). The crude extracts and isolated compounds were screened for in vitro antibacterial activity against strains of Klebsiella pneumoniae, Escherichia coli, Cryptococcus neoformans, Shigella dysentriae, and Staphylococcus aureus. Epicatechin (1) exhibited comparable antibacterial activity against S. aureus and E. coli (15 and 12 mm zone of inhibition, resp.) to that of the control antibiotic gentamicin, with zone of inhibition of 15 and 12 mm, respectively, at a concentration of 20 µg/mL. Hindawi 2018-04-30 /pmc/articles/PMC5952501/ /pubmed/29853867 http://dx.doi.org/10.1155/2018/5870161 Text en Copyright © 2018 Babe Guyasa et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Guyasa, Babe
Melaku, Yadessa
Endale, Milkyas
Antibacterial Activity of Two Flavans from the Stem Bark of Embelia schimperi
title Antibacterial Activity of Two Flavans from the Stem Bark of Embelia schimperi
title_full Antibacterial Activity of Two Flavans from the Stem Bark of Embelia schimperi
title_fullStr Antibacterial Activity of Two Flavans from the Stem Bark of Embelia schimperi
title_full_unstemmed Antibacterial Activity of Two Flavans from the Stem Bark of Embelia schimperi
title_short Antibacterial Activity of Two Flavans from the Stem Bark of Embelia schimperi
title_sort antibacterial activity of two flavans from the stem bark of embelia schimperi
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5952501/
https://www.ncbi.nlm.nih.gov/pubmed/29853867
http://dx.doi.org/10.1155/2018/5870161
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