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Identification of a flagellar protein implicated in the gravitaxis in the flagellate Euglena gracilis
Flagellated cells are of great evolutionary importance across animal and plant species. Unlike higher plants, flagellated cells are involved in reproduction of macro-algae as well as in early diverging land plants. Euglena gracilis is an emerging flagellated model organism. The current study reports...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5954063/ https://www.ncbi.nlm.nih.gov/pubmed/29765103 http://dx.doi.org/10.1038/s41598-018-26046-8 |
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author | Nasir, Adeel Le Bail, Aude Daiker, Viktor Klima, Janine Richter, Peter Lebert, Michael |
author_facet | Nasir, Adeel Le Bail, Aude Daiker, Viktor Klima, Janine Richter, Peter Lebert, Michael |
author_sort | Nasir, Adeel |
collection | PubMed |
description | Flagellated cells are of great evolutionary importance across animal and plant species. Unlike higher plants, flagellated cells are involved in reproduction of macro-algae as well as in early diverging land plants. Euglena gracilis is an emerging flagellated model organism. The current study reports that a specific calmodulin (CaM2) involved in gravitaxis of E. gracilis interacts with an evolutionary conserved flagellar protein, EgPCDUF4201. The subsequent molecular analysis showed clearly that EgPCDUF4201 is also involved in gravitaxis. We performed subcellular localization of CaM2 using immunoblotting and indirect immunofluorescence. By employing yeast two-hybrid screen, EgPCDUF4201 was identified as an interaction partner of CaM2. The C-terminus of EgPCDUF4201 is responsible for the interaction with CaM2. Silencing of N- and C-terminus of EgPCDUF4201 using RNAi resulted in an impaired gravitaxis. Moreover, indirect immunofluorescence assay showed that EgPCDUF4201 is a flagella associated protein. The current study specifically addressed some important questions regarding the signal transduction chain of gravitaxis in E. gracilis. Besides the fact that it improved the current understanding of gravity sensing mechanisms in E. gracilis, it also gave rise to several interesting research questions regarding the function of the domain of unknown function 4201 in flagellated cells. |
format | Online Article Text |
id | pubmed-5954063 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59540632018-05-21 Identification of a flagellar protein implicated in the gravitaxis in the flagellate Euglena gracilis Nasir, Adeel Le Bail, Aude Daiker, Viktor Klima, Janine Richter, Peter Lebert, Michael Sci Rep Article Flagellated cells are of great evolutionary importance across animal and plant species. Unlike higher plants, flagellated cells are involved in reproduction of macro-algae as well as in early diverging land plants. Euglena gracilis is an emerging flagellated model organism. The current study reports that a specific calmodulin (CaM2) involved in gravitaxis of E. gracilis interacts with an evolutionary conserved flagellar protein, EgPCDUF4201. The subsequent molecular analysis showed clearly that EgPCDUF4201 is also involved in gravitaxis. We performed subcellular localization of CaM2 using immunoblotting and indirect immunofluorescence. By employing yeast two-hybrid screen, EgPCDUF4201 was identified as an interaction partner of CaM2. The C-terminus of EgPCDUF4201 is responsible for the interaction with CaM2. Silencing of N- and C-terminus of EgPCDUF4201 using RNAi resulted in an impaired gravitaxis. Moreover, indirect immunofluorescence assay showed that EgPCDUF4201 is a flagella associated protein. The current study specifically addressed some important questions regarding the signal transduction chain of gravitaxis in E. gracilis. Besides the fact that it improved the current understanding of gravity sensing mechanisms in E. gracilis, it also gave rise to several interesting research questions regarding the function of the domain of unknown function 4201 in flagellated cells. Nature Publishing Group UK 2018-05-15 /pmc/articles/PMC5954063/ /pubmed/29765103 http://dx.doi.org/10.1038/s41598-018-26046-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nasir, Adeel Le Bail, Aude Daiker, Viktor Klima, Janine Richter, Peter Lebert, Michael Identification of a flagellar protein implicated in the gravitaxis in the flagellate Euglena gracilis |
title | Identification of a flagellar protein implicated in the gravitaxis in the flagellate Euglena gracilis |
title_full | Identification of a flagellar protein implicated in the gravitaxis in the flagellate Euglena gracilis |
title_fullStr | Identification of a flagellar protein implicated in the gravitaxis in the flagellate Euglena gracilis |
title_full_unstemmed | Identification of a flagellar protein implicated in the gravitaxis in the flagellate Euglena gracilis |
title_short | Identification of a flagellar protein implicated in the gravitaxis in the flagellate Euglena gracilis |
title_sort | identification of a flagellar protein implicated in the gravitaxis in the flagellate euglena gracilis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5954063/ https://www.ncbi.nlm.nih.gov/pubmed/29765103 http://dx.doi.org/10.1038/s41598-018-26046-8 |
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