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O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli

Extraintestinal pathogenic Escherichia coli (ExPEC) is the leading cause of bloodstream and other extraintestinal infections in human and animals. The greatest challenge encountered by ExPEC during an infection is posed by the host defense mechanisms, including lysozyme. ExPEC have developed diverse...

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Autores principales: Bao, Yinli, Zhang, Haobo, Huang, Xinxin, Ma, Jiale, Logue, Catherine M., Nolan, Lisa K., Li, Ganwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5955474/
https://www.ncbi.nlm.nih.gov/pubmed/29405825
http://dx.doi.org/10.1080/21505594.2018.1433979
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author Bao, Yinli
Zhang, Haobo
Huang, Xinxin
Ma, Jiale
Logue, Catherine M.
Nolan, Lisa K.
Li, Ganwu
author_facet Bao, Yinli
Zhang, Haobo
Huang, Xinxin
Ma, Jiale
Logue, Catherine M.
Nolan, Lisa K.
Li, Ganwu
author_sort Bao, Yinli
collection PubMed
description Extraintestinal pathogenic Escherichia coli (ExPEC) is the leading cause of bloodstream and other extraintestinal infections in human and animals. The greatest challenge encountered by ExPEC during an infection is posed by the host defense mechanisms, including lysozyme. ExPEC have developed diverse strategies to overcome this challenge. The aim of this study was to characterize the molecular mechanism of ExPEC resistance to lysozyme. For this, 15,000 transposon mutants of a lysozyme-resistant ExPEC strain NMEC38 were screened; 20 genes were identified as involved in ExPEC resistance to lysozyme—of which five were located in the gene cluster between galF and gnd, and were further confirmed to be involved in O-specific polysaccharide biosynthesis. The O-specific polysaccharide was able to inhibit the hydrolytic activity of lysozyme; it was also required by the complete lipopolysaccharide (LPS)-mediated protection of ExPEC against the bactericidal activity of lysozyme. The O-specific polysaccharide was further shown to be able to directly interact with lysozyme. Furthermore, LPS from ExPEC strains of different O serotypes was also able to inhibit the hydrolytic activity of lysozyme. Because of their cell surface localization and wide distribution in Gram-negative bacteria, O-specific polysaccharides appear to play a long-overlooked role in protecting bacteria against exogenous lysozyme.
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spelling pubmed-59554742018-05-21 O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli Bao, Yinli Zhang, Haobo Huang, Xinxin Ma, Jiale Logue, Catherine M. Nolan, Lisa K. Li, Ganwu Virulence Research Paper Extraintestinal pathogenic Escherichia coli (ExPEC) is the leading cause of bloodstream and other extraintestinal infections in human and animals. The greatest challenge encountered by ExPEC during an infection is posed by the host defense mechanisms, including lysozyme. ExPEC have developed diverse strategies to overcome this challenge. The aim of this study was to characterize the molecular mechanism of ExPEC resistance to lysozyme. For this, 15,000 transposon mutants of a lysozyme-resistant ExPEC strain NMEC38 were screened; 20 genes were identified as involved in ExPEC resistance to lysozyme—of which five were located in the gene cluster between galF and gnd, and were further confirmed to be involved in O-specific polysaccharide biosynthesis. The O-specific polysaccharide was able to inhibit the hydrolytic activity of lysozyme; it was also required by the complete lipopolysaccharide (LPS)-mediated protection of ExPEC against the bactericidal activity of lysozyme. The O-specific polysaccharide was further shown to be able to directly interact with lysozyme. Furthermore, LPS from ExPEC strains of different O serotypes was also able to inhibit the hydrolytic activity of lysozyme. Because of their cell surface localization and wide distribution in Gram-negative bacteria, O-specific polysaccharides appear to play a long-overlooked role in protecting bacteria against exogenous lysozyme. Taylor & Francis 2018-03-19 /pmc/articles/PMC5955474/ /pubmed/29405825 http://dx.doi.org/10.1080/21505594.2018.1433979 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Bao, Yinli
Zhang, Haobo
Huang, Xinxin
Ma, Jiale
Logue, Catherine M.
Nolan, Lisa K.
Li, Ganwu
O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli
title O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli
title_full O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli
title_fullStr O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli
title_full_unstemmed O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli
title_short O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli
title_sort o-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic escherichia coli
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5955474/
https://www.ncbi.nlm.nih.gov/pubmed/29405825
http://dx.doi.org/10.1080/21505594.2018.1433979
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