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Establishment of an Alzheimer’s disease model with latent herpesvirus infection using PS2 and Tg2576 double transgenic mice
A relationship between Alzheimer’s disease and herpes simplex virus infection has been pointed out. We established a model of Alzheimer’s disease with a latent herpesvirus infection using a mouse model of Alzheimer’s disease (PS2Tg2576) and examined the changes in amyloid β (Aβ) in the brain. We cro...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Japanese Association for Laboratory Animal Science
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5955750/ https://www.ncbi.nlm.nih.gov/pubmed/29187699 http://dx.doi.org/10.1538/expanim.17-0066 |
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author | Tanaka, Seiichi Nagashima, Hiroshi |
author_facet | Tanaka, Seiichi Nagashima, Hiroshi |
author_sort | Tanaka, Seiichi |
collection | PubMed |
description | A relationship between Alzheimer’s disease and herpes simplex virus infection has been pointed out. We established a model of Alzheimer’s disease with a latent herpesvirus infection using a mouse model of Alzheimer’s disease (PS2Tg2576) and examined the changes in amyloid β (Aβ) in the brain. We crossbred female PS2 mice with male Tg2576 hemi mice and chose PS2Tg2576 mice. After priming 5-week-old male mice with anti-pseudorabies virus swine serum, we challenged the mouse with 100 LD(50) of YS-81, a wild-type strain of pseudorabies virus. The viral DNA was detected in nasal swabs by a reactivation test and in the trigeminal ganglia. At two months after infection, the Aβ40 and Aβ42 levels in the brains of the mice of the latently infected group were increased; the increase was greater than that observed in the noninfected group. Latent pseudorabies virus infection was established in PS2Tg2576 mice and the level of Aβ increased with the reactivation of the latent virus. |
format | Online Article Text |
id | pubmed-5955750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Japanese Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-59557502018-05-21 Establishment of an Alzheimer’s disease model with latent herpesvirus infection using PS2 and Tg2576 double transgenic mice Tanaka, Seiichi Nagashima, Hiroshi Exp Anim Original A relationship between Alzheimer’s disease and herpes simplex virus infection has been pointed out. We established a model of Alzheimer’s disease with a latent herpesvirus infection using a mouse model of Alzheimer’s disease (PS2Tg2576) and examined the changes in amyloid β (Aβ) in the brain. We crossbred female PS2 mice with male Tg2576 hemi mice and chose PS2Tg2576 mice. After priming 5-week-old male mice with anti-pseudorabies virus swine serum, we challenged the mouse with 100 LD(50) of YS-81, a wild-type strain of pseudorabies virus. The viral DNA was detected in nasal swabs by a reactivation test and in the trigeminal ganglia. At two months after infection, the Aβ40 and Aβ42 levels in the brains of the mice of the latently infected group were increased; the increase was greater than that observed in the noninfected group. Latent pseudorabies virus infection was established in PS2Tg2576 mice and the level of Aβ increased with the reactivation of the latent virus. Japanese Association for Laboratory Animal Science 2017-11-29 2018 /pmc/articles/PMC5955750/ /pubmed/29187699 http://dx.doi.org/10.1538/expanim.17-0066 Text en ©2018 Japanese Association for Laboratory Animal Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Original Tanaka, Seiichi Nagashima, Hiroshi Establishment of an Alzheimer’s disease model with latent herpesvirus infection using PS2 and Tg2576 double transgenic mice |
title | Establishment of an Alzheimer’s disease model with latent herpesvirus
infection using PS2 and Tg2576 double transgenic mice |
title_full | Establishment of an Alzheimer’s disease model with latent herpesvirus
infection using PS2 and Tg2576 double transgenic mice |
title_fullStr | Establishment of an Alzheimer’s disease model with latent herpesvirus
infection using PS2 and Tg2576 double transgenic mice |
title_full_unstemmed | Establishment of an Alzheimer’s disease model with latent herpesvirus
infection using PS2 and Tg2576 double transgenic mice |
title_short | Establishment of an Alzheimer’s disease model with latent herpesvirus
infection using PS2 and Tg2576 double transgenic mice |
title_sort | establishment of an alzheimer’s disease model with latent herpesvirus
infection using ps2 and tg2576 double transgenic mice |
topic | Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5955750/ https://www.ncbi.nlm.nih.gov/pubmed/29187699 http://dx.doi.org/10.1538/expanim.17-0066 |
work_keys_str_mv | AT tanakaseiichi establishmentofanalzheimersdiseasemodelwithlatentherpesvirusinfectionusingps2andtg2576doubletransgenicmice AT nagashimahiroshi establishmentofanalzheimersdiseasemodelwithlatentherpesvirusinfectionusingps2andtg2576doubletransgenicmice |