Cargando…

Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound

Reactive oxygen and nitrogen species released by cold physical plasma are being proposed as effectors in various clinical conditions connected to inflammatory processes. As these plasmas can be tailored in a wide range, models to compare and control their biochemical footprint are desired to infer o...

Descripción completa

Detalles Bibliográficos
Autores principales: Lackmann, J.-W., Wende, K., Verlackt, C., Golda, J., Volzke, J., Kogelheide, F., Held, J., Bekeschus, S., Bogaerts, A., Schulz-von der Gathen, V., Stapelmann, K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5955931/
https://www.ncbi.nlm.nih.gov/pubmed/29769633
http://dx.doi.org/10.1038/s41598-018-25937-0
_version_ 1783323791672213504
author Lackmann, J.-W.
Wende, K.
Verlackt, C.
Golda, J.
Volzke, J.
Kogelheide, F.
Held, J.
Bekeschus, S.
Bogaerts, A.
Schulz-von der Gathen, V.
Stapelmann, K.
author_facet Lackmann, J.-W.
Wende, K.
Verlackt, C.
Golda, J.
Volzke, J.
Kogelheide, F.
Held, J.
Bekeschus, S.
Bogaerts, A.
Schulz-von der Gathen, V.
Stapelmann, K.
author_sort Lackmann, J.-W.
collection PubMed
description Reactive oxygen and nitrogen species released by cold physical plasma are being proposed as effectors in various clinical conditions connected to inflammatory processes. As these plasmas can be tailored in a wide range, models to compare and control their biochemical footprint are desired to infer on the molecular mechanisms underlying the observed effects and to enable the discrimination between different plasma sources. Here, an improved model to trace short-lived reactive species is presented. Using FTIR, high-resolution mass spectrometry, and molecular dynamics computational simulation, covalent modifications of cysteine treated with different plasmas were deciphered and the respective product pattern used to generate a fingerprint of each plasma source. Such, our experimental model allows a fast and reliable grading of the chemical potential of plasmas used for medical purposes. Major reaction products were identified to be cysteine sulfonic acid, cystine, and cysteine fragments. Less-abundant products, such as oxidized cystine derivatives or S-nitrosylated cysteines, were unique to different plasma sources or operating conditions. The data collected point at hydroxyl radicals, atomic O, and singlet oxygen as major contributing species that enable an impact on cellular thiol groups when applying cold plasma in vitro or in vivo.
format Online
Article
Text
id pubmed-5955931
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-59559312018-05-21 Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound Lackmann, J.-W. Wende, K. Verlackt, C. Golda, J. Volzke, J. Kogelheide, F. Held, J. Bekeschus, S. Bogaerts, A. Schulz-von der Gathen, V. Stapelmann, K. Sci Rep Article Reactive oxygen and nitrogen species released by cold physical plasma are being proposed as effectors in various clinical conditions connected to inflammatory processes. As these plasmas can be tailored in a wide range, models to compare and control their biochemical footprint are desired to infer on the molecular mechanisms underlying the observed effects and to enable the discrimination between different plasma sources. Here, an improved model to trace short-lived reactive species is presented. Using FTIR, high-resolution mass spectrometry, and molecular dynamics computational simulation, covalent modifications of cysteine treated with different plasmas were deciphered and the respective product pattern used to generate a fingerprint of each plasma source. Such, our experimental model allows a fast and reliable grading of the chemical potential of plasmas used for medical purposes. Major reaction products were identified to be cysteine sulfonic acid, cystine, and cysteine fragments. Less-abundant products, such as oxidized cystine derivatives or S-nitrosylated cysteines, were unique to different plasma sources or operating conditions. The data collected point at hydroxyl radicals, atomic O, and singlet oxygen as major contributing species that enable an impact on cellular thiol groups when applying cold plasma in vitro or in vivo. Nature Publishing Group UK 2018-05-16 /pmc/articles/PMC5955931/ /pubmed/29769633 http://dx.doi.org/10.1038/s41598-018-25937-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lackmann, J.-W.
Wende, K.
Verlackt, C.
Golda, J.
Volzke, J.
Kogelheide, F.
Held, J.
Bekeschus, S.
Bogaerts, A.
Schulz-von der Gathen, V.
Stapelmann, K.
Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound
title Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound
title_full Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound
title_fullStr Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound
title_full_unstemmed Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound
title_short Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound
title_sort chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5955931/
https://www.ncbi.nlm.nih.gov/pubmed/29769633
http://dx.doi.org/10.1038/s41598-018-25937-0
work_keys_str_mv AT lackmannjw chemicalfingerprintsofcoldphysicalplasmasanexperimentalandcomputationalstudyusingcysteineastracercompound
AT wendek chemicalfingerprintsofcoldphysicalplasmasanexperimentalandcomputationalstudyusingcysteineastracercompound
AT verlacktc chemicalfingerprintsofcoldphysicalplasmasanexperimentalandcomputationalstudyusingcysteineastracercompound
AT goldaj chemicalfingerprintsofcoldphysicalplasmasanexperimentalandcomputationalstudyusingcysteineastracercompound
AT volzkej chemicalfingerprintsofcoldphysicalplasmasanexperimentalandcomputationalstudyusingcysteineastracercompound
AT kogelheidef chemicalfingerprintsofcoldphysicalplasmasanexperimentalandcomputationalstudyusingcysteineastracercompound
AT heldj chemicalfingerprintsofcoldphysicalplasmasanexperimentalandcomputationalstudyusingcysteineastracercompound
AT bekeschuss chemicalfingerprintsofcoldphysicalplasmasanexperimentalandcomputationalstudyusingcysteineastracercompound
AT bogaertsa chemicalfingerprintsofcoldphysicalplasmasanexperimentalandcomputationalstudyusingcysteineastracercompound
AT schulzvondergathenv chemicalfingerprintsofcoldphysicalplasmasanexperimentalandcomputationalstudyusingcysteineastracercompound
AT stapelmannk chemicalfingerprintsofcoldphysicalplasmasanexperimentalandcomputationalstudyusingcysteineastracercompound