A co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture

A meniscus tear often happens during active sports. It needs to be repaired or replaced surgically to avoid further damage to the articular cartilage. To address the shortage of autologous meniscal cells, we designed a co-culture system of synovial stem cells (SMSCs) and meniscal cells (MCs) to prod...

Descripción completa

Detalles Bibliográficos
Autores principales: Xie, Xing, Zhu, Jingxian, Hu, Xiaoqing, Dai, Linghui, Fu, Xin, Zhang, Jiying, Duan, Xiaoning, Ao, Yingfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5955983/
https://www.ncbi.nlm.nih.gov/pubmed/29769537
http://dx.doi.org/10.1038/s41598-018-25709-w
_version_ 1783323803520073728
author Xie, Xing
Zhu, Jingxian
Hu, Xiaoqing
Dai, Linghui
Fu, Xin
Zhang, Jiying
Duan, Xiaoning
Ao, Yingfang
author_facet Xie, Xing
Zhu, Jingxian
Hu, Xiaoqing
Dai, Linghui
Fu, Xin
Zhang, Jiying
Duan, Xiaoning
Ao, Yingfang
author_sort Xie, Xing
collection PubMed
description A meniscus tear often happens during active sports. It needs to be repaired or replaced surgically to avoid further damage to the articular cartilage. To address the shortage of autologous meniscal cells, we designed a co-culture system of synovial stem cells (SMSCs) and meniscal cells (MCs) to produce a large cell number and to maintain characteristics of MCs. Different ratios of SMSCs and MCs at 3:1, 1:1, and 1:3 were tested. Mono-culture of SMSCs or MCs served as control groups. Proliferation and differentiation abilities were compared. The expression of extracellular matrix (ECM) genes in MCs was assessed using an ECM array to reveal the mechanism at the gene level. The co-culture system of SMSCs/MCs at the ratio of 1:3 showed better results than the control groups or those at other ratios. This co-culture system may be a promising strategy for meniscus repair with tissue engineering.
format Online
Article
Text
id pubmed-5955983
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-59559832018-05-21 A co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture Xie, Xing Zhu, Jingxian Hu, Xiaoqing Dai, Linghui Fu, Xin Zhang, Jiying Duan, Xiaoning Ao, Yingfang Sci Rep Article A meniscus tear often happens during active sports. It needs to be repaired or replaced surgically to avoid further damage to the articular cartilage. To address the shortage of autologous meniscal cells, we designed a co-culture system of synovial stem cells (SMSCs) and meniscal cells (MCs) to produce a large cell number and to maintain characteristics of MCs. Different ratios of SMSCs and MCs at 3:1, 1:1, and 1:3 were tested. Mono-culture of SMSCs or MCs served as control groups. Proliferation and differentiation abilities were compared. The expression of extracellular matrix (ECM) genes in MCs was assessed using an ECM array to reveal the mechanism at the gene level. The co-culture system of SMSCs/MCs at the ratio of 1:3 showed better results than the control groups or those at other ratios. This co-culture system may be a promising strategy for meniscus repair with tissue engineering. Nature Publishing Group UK 2018-05-16 /pmc/articles/PMC5955983/ /pubmed/29769537 http://dx.doi.org/10.1038/s41598-018-25709-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xie, Xing
Zhu, Jingxian
Hu, Xiaoqing
Dai, Linghui
Fu, Xin
Zhang, Jiying
Duan, Xiaoning
Ao, Yingfang
A co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture
title A co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture
title_full A co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture
title_fullStr A co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture
title_full_unstemmed A co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture
title_short A co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture
title_sort co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5955983/
https://www.ncbi.nlm.nih.gov/pubmed/29769537
http://dx.doi.org/10.1038/s41598-018-25709-w
work_keys_str_mv AT xiexing acoculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT zhujingxian acoculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT huxiaoqing acoculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT dailinghui acoculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT fuxin acoculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT zhangjiying acoculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT duanxiaoning acoculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT aoyingfang acoculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT xiexing coculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT zhujingxian coculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT huxiaoqing coculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT dailinghui coculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT fuxin coculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT zhangjiying coculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT duanxiaoning coculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture
AT aoyingfang coculturesystemofratsynovialstemcellsandmeniscuscellspromotescellproliferationanddifferentiationascomparedtomonoculture

Ejemplares similares