The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons

Neural stem cells (NSCs) are self-renewing multipotent stem cells that can be proliferated in vitro and differentiated into neuronal and/or glial lineages, making them an ideal model to study the processes involved in neuronal differentiation. Here we have used NSCs to investigate the role of the tr...

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Autores principales: Zhu, Bangfu, Carmichael, Ruth E., Solabre Valois, Luis, Wilkinson, Kevin A., Henley, Jeremy M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5956278/
https://www.ncbi.nlm.nih.gov/pubmed/29678571
http://dx.doi.org/10.1016/j.bbrc.2018.04.125
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author Zhu, Bangfu
Carmichael, Ruth E.
Solabre Valois, Luis
Wilkinson, Kevin A.
Henley, Jeremy M.
author_facet Zhu, Bangfu
Carmichael, Ruth E.
Solabre Valois, Luis
Wilkinson, Kevin A.
Henley, Jeremy M.
author_sort Zhu, Bangfu
collection PubMed
description Neural stem cells (NSCs) are self-renewing multipotent stem cells that can be proliferated in vitro and differentiated into neuronal and/or glial lineages, making them an ideal model to study the processes involved in neuronal differentiation. Here we have used NSCs to investigate the role of the transcription factor MEF2A in neuronal differentiation and development in vitro. We show that although MEF2A is present in undifferentiated NSCs, following differentiation it is expressed at significantly higher levels in a subset of neuronal compared to non-neuronal cells. Furthermore, shRNA-mediated knockdown of MEF2A reduces the number of NSC-derived neurons compared to non-neuronal cells after differentiation. Together, these data indicate that MEF2A participates in neuronal differentiation/maturation from NSCs.
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spelling pubmed-59562782018-06-07 The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons Zhu, Bangfu Carmichael, Ruth E. Solabre Valois, Luis Wilkinson, Kevin A. Henley, Jeremy M. Biochem Biophys Res Commun Article Neural stem cells (NSCs) are self-renewing multipotent stem cells that can be proliferated in vitro and differentiated into neuronal and/or glial lineages, making them an ideal model to study the processes involved in neuronal differentiation. Here we have used NSCs to investigate the role of the transcription factor MEF2A in neuronal differentiation and development in vitro. We show that although MEF2A is present in undifferentiated NSCs, following differentiation it is expressed at significantly higher levels in a subset of neuronal compared to non-neuronal cells. Furthermore, shRNA-mediated knockdown of MEF2A reduces the number of NSC-derived neurons compared to non-neuronal cells after differentiation. Together, these data indicate that MEF2A participates in neuronal differentiation/maturation from NSCs. Elsevier 2018-06-07 /pmc/articles/PMC5956278/ /pubmed/29678571 http://dx.doi.org/10.1016/j.bbrc.2018.04.125 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhu, Bangfu
Carmichael, Ruth E.
Solabre Valois, Luis
Wilkinson, Kevin A.
Henley, Jeremy M.
The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons
title The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons
title_full The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons
title_fullStr The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons
title_full_unstemmed The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons
title_short The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons
title_sort transcription factor mef2a plays a key role in the differentiation/maturation of rat neural stem cells into neurons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5956278/
https://www.ncbi.nlm.nih.gov/pubmed/29678571
http://dx.doi.org/10.1016/j.bbrc.2018.04.125
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