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Salt mine microorganisms used for the biotransformation of chlorolactones

The aim of the project was to find new catalysts capable of chlorolactone biotransformation. Three bicyclic chlorolactones with structures possessing one or two methyl groups in their cyclohexane ring were subjected to screening biotransformation using seven bacterial strains and one fungal strain f...

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Autores principales: Mączka, Wanda, Grabarczyk, Małgorzata, Wińska, Katarzyna, Gębarowska, Elżbieta, Strzała, Tomasz, Durajczyk, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5957361/
https://www.ncbi.nlm.nih.gov/pubmed/29771957
http://dx.doi.org/10.1371/journal.pone.0197384
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author Mączka, Wanda
Grabarczyk, Małgorzata
Wińska, Katarzyna
Gębarowska, Elżbieta
Strzała, Tomasz
Durajczyk, Marek
author_facet Mączka, Wanda
Grabarczyk, Małgorzata
Wińska, Katarzyna
Gębarowska, Elżbieta
Strzała, Tomasz
Durajczyk, Marek
author_sort Mączka, Wanda
collection PubMed
description The aim of the project was to find new catalysts capable of chlorolactone biotransformation. Three bicyclic chlorolactones with structures possessing one or two methyl groups in their cyclohexane ring were subjected to screening biotransformation using seven bacterial strains and one fungal strain from a salt mine. Three strains of bacteria (Micrococcus luteus Pb10, Micrococcus luteus WSP45, Gordonia alkanivorans Pd25) and one fungal strain (Aspergillus sydowii KGJ10) were able to catalyse hydrolytic dehalogenation of one substrate. The classification of the strains that were effective biocatalysts was confirmed by 16S rDNA analysis. The best result (76%) was obtained using Aspergillus sydowii KGJ10. All strains catalysed hydrolytic dehalogenation without changing the conformation. The equatorial position of the chlorine atom in the substrate turned out to be warrant of the positive result of the biotransformation process.
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spelling pubmed-59573612018-05-31 Salt mine microorganisms used for the biotransformation of chlorolactones Mączka, Wanda Grabarczyk, Małgorzata Wińska, Katarzyna Gębarowska, Elżbieta Strzała, Tomasz Durajczyk, Marek PLoS One Research Article The aim of the project was to find new catalysts capable of chlorolactone biotransformation. Three bicyclic chlorolactones with structures possessing one or two methyl groups in their cyclohexane ring were subjected to screening biotransformation using seven bacterial strains and one fungal strain from a salt mine. Three strains of bacteria (Micrococcus luteus Pb10, Micrococcus luteus WSP45, Gordonia alkanivorans Pd25) and one fungal strain (Aspergillus sydowii KGJ10) were able to catalyse hydrolytic dehalogenation of one substrate. The classification of the strains that were effective biocatalysts was confirmed by 16S rDNA analysis. The best result (76%) was obtained using Aspergillus sydowii KGJ10. All strains catalysed hydrolytic dehalogenation without changing the conformation. The equatorial position of the chlorine atom in the substrate turned out to be warrant of the positive result of the biotransformation process. Public Library of Science 2018-05-17 /pmc/articles/PMC5957361/ /pubmed/29771957 http://dx.doi.org/10.1371/journal.pone.0197384 Text en © 2018 Mączka et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mączka, Wanda
Grabarczyk, Małgorzata
Wińska, Katarzyna
Gębarowska, Elżbieta
Strzała, Tomasz
Durajczyk, Marek
Salt mine microorganisms used for the biotransformation of chlorolactones
title Salt mine microorganisms used for the biotransformation of chlorolactones
title_full Salt mine microorganisms used for the biotransformation of chlorolactones
title_fullStr Salt mine microorganisms used for the biotransformation of chlorolactones
title_full_unstemmed Salt mine microorganisms used for the biotransformation of chlorolactones
title_short Salt mine microorganisms used for the biotransformation of chlorolactones
title_sort salt mine microorganisms used for the biotransformation of chlorolactones
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5957361/
https://www.ncbi.nlm.nih.gov/pubmed/29771957
http://dx.doi.org/10.1371/journal.pone.0197384
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