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Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum
A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD(4)) was constructed. Intact nuclei from G. mustelinum (AD(4)) were used to isolate high molecular weight DNA, which was partially cleaved with Hind III and cloned into pSMART BAC (Hind III) vectors. The BAC librar...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5957370/ https://www.ncbi.nlm.nih.gov/pubmed/29771937 http://dx.doi.org/10.1371/journal.pone.0196847 |
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author | Liu, Yuling Zhang, Baohong Wen, Xinpeng Zhang, Shulin Wei, Yangyang Lu, Quanwei Liu, Zhen Wang, Kunbo Liu, Fang Peng, Renhai |
author_facet | Liu, Yuling Zhang, Baohong Wen, Xinpeng Zhang, Shulin Wei, Yangyang Lu, Quanwei Liu, Zhen Wang, Kunbo Liu, Fang Peng, Renhai |
author_sort | Liu, Yuling |
collection | PubMed |
description | A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD(4)) was constructed. Intact nuclei from G. mustelinum (AD(4)) were used to isolate high molecular weight DNA, which was partially cleaved with Hind III and cloned into pSMART BAC (Hind III) vectors. The BAC library consisted of 208,182 clones arrayed in 542 384-microtiter plates, with an average insert size of 121.72 kb ranging from 100 to 150 kb. About 2% of the clones did not contain inserts. Based on an estimated genome size of 2372 Mb for G. mustelinum, the BAC library was estimated to have a total coverage of 10.50 × genome equivalents. The high capacity library of G. mustelinum will serve as a giant gene resource for map-based cloning of quantitative trait loci or genes associated with important agronomic traits or resistance to Verticillium wilt, physical mapping and comparative genome analysis. |
format | Online Article Text |
id | pubmed-5957370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-59573702018-05-31 Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum Liu, Yuling Zhang, Baohong Wen, Xinpeng Zhang, Shulin Wei, Yangyang Lu, Quanwei Liu, Zhen Wang, Kunbo Liu, Fang Peng, Renhai PLoS One Research Article A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD(4)) was constructed. Intact nuclei from G. mustelinum (AD(4)) were used to isolate high molecular weight DNA, which was partially cleaved with Hind III and cloned into pSMART BAC (Hind III) vectors. The BAC library consisted of 208,182 clones arrayed in 542 384-microtiter plates, with an average insert size of 121.72 kb ranging from 100 to 150 kb. About 2% of the clones did not contain inserts. Based on an estimated genome size of 2372 Mb for G. mustelinum, the BAC library was estimated to have a total coverage of 10.50 × genome equivalents. The high capacity library of G. mustelinum will serve as a giant gene resource for map-based cloning of quantitative trait loci or genes associated with important agronomic traits or resistance to Verticillium wilt, physical mapping and comparative genome analysis. Public Library of Science 2018-05-17 /pmc/articles/PMC5957370/ /pubmed/29771937 http://dx.doi.org/10.1371/journal.pone.0196847 Text en © 2018 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Liu, Yuling Zhang, Baohong Wen, Xinpeng Zhang, Shulin Wei, Yangyang Lu, Quanwei Liu, Zhen Wang, Kunbo Liu, Fang Peng, Renhai Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum |
title | Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum |
title_full | Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum |
title_fullStr | Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum |
title_full_unstemmed | Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum |
title_short | Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum |
title_sort | construction and characterization of a bacterial artificial chromosome library for gossypium mustelinum |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5957370/ https://www.ncbi.nlm.nih.gov/pubmed/29771937 http://dx.doi.org/10.1371/journal.pone.0196847 |
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