RNase H-assisted RNA-primed rolling circle amplification for targeted RNA sequence detection

RNA-primed rolling circle amplification (RPRCA) is a useful laboratory method for RNA detection; however, the detection of RNA is limited by the lack of information on 3′-terminal sequences. We uncovered that conventional RPRCA using pre-circularized probes could potentially detect the internal sequ...

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Detalles Bibliográficos
Autores principales: Takahashi, Hirokazu, Ohkawachi, Masahiko, Horio, Kyohei, Kobori, Toshiro, Aki, Tsunehiro, Matsumura, Yukihiko, Nakashimada, Yutaka, Okamura, Yoshiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958062/
https://www.ncbi.nlm.nih.gov/pubmed/29773824
http://dx.doi.org/10.1038/s41598-018-26132-x

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