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Transcriptome and miRNA analyses of the response to Corynespora cassiicola in cucumber
Cucumber (Cucumis sativus L.) target leaf spot (TLS), which is caused by the fungus Corynespora cassiicola (C. cassiicola), seriously endangers the production of cucumber. In this assay, we performed comprehensive sequencing of the transcriptome and microRNAs (miRNAs) of a resistant cucumber (Jinyou...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958113/ https://www.ncbi.nlm.nih.gov/pubmed/29773833 http://dx.doi.org/10.1038/s41598-018-26080-6 |
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author | Wang, Xiangyu Zhang, Di Cui, Na Yu, Yang Yu, Guangchao Fan, Haiyan |
author_facet | Wang, Xiangyu Zhang, Di Cui, Na Yu, Yang Yu, Guangchao Fan, Haiyan |
author_sort | Wang, Xiangyu |
collection | PubMed |
description | Cucumber (Cucumis sativus L.) target leaf spot (TLS), which is caused by the fungus Corynespora cassiicola (C. cassiicola), seriously endangers the production of cucumber. In this assay, we performed comprehensive sequencing of the transcriptome and microRNAs (miRNAs) of a resistant cucumber (Jinyou 38) during C. cassiicola inoculation using the Illumina NextSeq 500 platform. The possible genes related to the response to C. cassiicola were associated with plant hormones, transcription factors, primary metabolism, Ca(2+) signaling pathways, secondary metabolism and defense genes. In total, 150 target genes of these differentially expressed miRNAs were predicted by the bioinformatic analysis. By analyzing the function of the target genes, several candidate miRNAs that may be related to the response to C. cassiicola stress were selected. We also predicted 7 novel miRNAs and predicted their target genes. Moreover, the expression patterns of the candidate genes and miRNAs were tested by quantitative real-time RT-PCR. According to the analysis, genes and miRNAs associated with secondary metabolism, particularly the phenylpropanoid biosynthesis pathway, may play a major role in the resistance to C. cassiicola stress in cucumber. These results offer a foundation for future studies exploring the mechanism and key genes of resistance to cucumber TLS. |
format | Online Article Text |
id | pubmed-5958113 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59581132018-05-24 Transcriptome and miRNA analyses of the response to Corynespora cassiicola in cucumber Wang, Xiangyu Zhang, Di Cui, Na Yu, Yang Yu, Guangchao Fan, Haiyan Sci Rep Article Cucumber (Cucumis sativus L.) target leaf spot (TLS), which is caused by the fungus Corynespora cassiicola (C. cassiicola), seriously endangers the production of cucumber. In this assay, we performed comprehensive sequencing of the transcriptome and microRNAs (miRNAs) of a resistant cucumber (Jinyou 38) during C. cassiicola inoculation using the Illumina NextSeq 500 platform. The possible genes related to the response to C. cassiicola were associated with plant hormones, transcription factors, primary metabolism, Ca(2+) signaling pathways, secondary metabolism and defense genes. In total, 150 target genes of these differentially expressed miRNAs were predicted by the bioinformatic analysis. By analyzing the function of the target genes, several candidate miRNAs that may be related to the response to C. cassiicola stress were selected. We also predicted 7 novel miRNAs and predicted their target genes. Moreover, the expression patterns of the candidate genes and miRNAs were tested by quantitative real-time RT-PCR. According to the analysis, genes and miRNAs associated with secondary metabolism, particularly the phenylpropanoid biosynthesis pathway, may play a major role in the resistance to C. cassiicola stress in cucumber. These results offer a foundation for future studies exploring the mechanism and key genes of resistance to cucumber TLS. Nature Publishing Group UK 2018-05-17 /pmc/articles/PMC5958113/ /pubmed/29773833 http://dx.doi.org/10.1038/s41598-018-26080-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wang, Xiangyu Zhang, Di Cui, Na Yu, Yang Yu, Guangchao Fan, Haiyan Transcriptome and miRNA analyses of the response to Corynespora cassiicola in cucumber |
title | Transcriptome and miRNA analyses of the response to Corynespora cassiicola in cucumber |
title_full | Transcriptome and miRNA analyses of the response to Corynespora cassiicola in cucumber |
title_fullStr | Transcriptome and miRNA analyses of the response to Corynespora cassiicola in cucumber |
title_full_unstemmed | Transcriptome and miRNA analyses of the response to Corynespora cassiicola in cucumber |
title_short | Transcriptome and miRNA analyses of the response to Corynespora cassiicola in cucumber |
title_sort | transcriptome and mirna analyses of the response to corynespora cassiicola in cucumber |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958113/ https://www.ncbi.nlm.nih.gov/pubmed/29773833 http://dx.doi.org/10.1038/s41598-018-26080-6 |
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