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Hypermethylation of MDFI promoter with NSCLC is specific for females, non-smokers and people younger than 65
Non-small cell lung carcinoma (NSCLC) is a major subtype of lung cancer. Aberrant DNA methylation has been frequently observed in NSCLC. The aim of the present study was to investigate the role of MyoD family inhibitor (MDFI) methylation in NSCLC. Formalin-fixed paraffin-embedded tumor tissues and a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958687/ https://www.ncbi.nlm.nih.gov/pubmed/29805634 http://dx.doi.org/10.3892/ol.2018.8535 |
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author | Ma, Hongying Chen, Xiaoying Hu, Haochang Li, Bin Ying, Xiuru Zhou, Cong Zhong, Jie Zhao, Guofang Duan, Shiwei |
author_facet | Ma, Hongying Chen, Xiaoying Hu, Haochang Li, Bin Ying, Xiuru Zhou, Cong Zhong, Jie Zhao, Guofang Duan, Shiwei |
author_sort | Ma, Hongying |
collection | PubMed |
description | Non-small cell lung carcinoma (NSCLC) is a major subtype of lung cancer. Aberrant DNA methylation has been frequently observed in NSCLC. The aim of the present study was to investigate the role of MyoD family inhibitor (MDFI) methylation in NSCLC. Formalin-fixed paraffin-embedded tumor tissues and adjacent non-cancerous tissues were collected from a total of 111 patients with NSCLC. A methylation assay was performed using the quantitative methylation-specific polymerase chain reaction method. The percentage of methylated reference was used to represent the methylation level of the MDFI promoter. Data mining of a dataset from The Cancer Genome Atlas (TCGA) demonstrated that MDFI promoter methylation levels were significantly increased in 830 tumor tissues compared with 75 non-tumor tissues (P=0.012). However, the results on tissues obtained in the present study indicated that the MDFI promoter methylation levels in tumor tissues were not significantly different compared with those in the adjacent non-tumor tissues (P=0.159). Subsequent breakdown analysis identified that higher MDFI promoter methylation levels were significantly associated with NSCLC in females (P=0.031), but not in males (P=0.832). Age-based subgroup analysis demonstrated that higher MDFI promoter methylation levels were significantly associated with NSCLC in younger patients (≤65 years; P=0.003), but not in older patients (P=0.327). In addition, the association of MDFI methylation with NSCLC was significant in non-smokers (P=0.014), but not in smokers (P=0.832). Similar results also have been determined from subgroup analysis of the TCGA datasets. The Gene Expression Omnibus database indicated MDFI expression restoration in partial lung cancer cell lines (H1299 and Hotz) following demethylation treatment. However, it was identified that MDFI promoter hypermethylation was not significantly associated with prognosis of NSCLC (P>0.05). In conclusion, the present study indicated that the association of higher methylation of the MDFI promoter with NSCLC may be specific to females, non-smokers and people aged ≤65. |
format | Online Article Text |
id | pubmed-5958687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-59586872018-05-27 Hypermethylation of MDFI promoter with NSCLC is specific for females, non-smokers and people younger than 65 Ma, Hongying Chen, Xiaoying Hu, Haochang Li, Bin Ying, Xiuru Zhou, Cong Zhong, Jie Zhao, Guofang Duan, Shiwei Oncol Lett Articles Non-small cell lung carcinoma (NSCLC) is a major subtype of lung cancer. Aberrant DNA methylation has been frequently observed in NSCLC. The aim of the present study was to investigate the role of MyoD family inhibitor (MDFI) methylation in NSCLC. Formalin-fixed paraffin-embedded tumor tissues and adjacent non-cancerous tissues were collected from a total of 111 patients with NSCLC. A methylation assay was performed using the quantitative methylation-specific polymerase chain reaction method. The percentage of methylated reference was used to represent the methylation level of the MDFI promoter. Data mining of a dataset from The Cancer Genome Atlas (TCGA) demonstrated that MDFI promoter methylation levels were significantly increased in 830 tumor tissues compared with 75 non-tumor tissues (P=0.012). However, the results on tissues obtained in the present study indicated that the MDFI promoter methylation levels in tumor tissues were not significantly different compared with those in the adjacent non-tumor tissues (P=0.159). Subsequent breakdown analysis identified that higher MDFI promoter methylation levels were significantly associated with NSCLC in females (P=0.031), but not in males (P=0.832). Age-based subgroup analysis demonstrated that higher MDFI promoter methylation levels were significantly associated with NSCLC in younger patients (≤65 years; P=0.003), but not in older patients (P=0.327). In addition, the association of MDFI methylation with NSCLC was significant in non-smokers (P=0.014), but not in smokers (P=0.832). Similar results also have been determined from subgroup analysis of the TCGA datasets. The Gene Expression Omnibus database indicated MDFI expression restoration in partial lung cancer cell lines (H1299 and Hotz) following demethylation treatment. However, it was identified that MDFI promoter hypermethylation was not significantly associated with prognosis of NSCLC (P>0.05). In conclusion, the present study indicated that the association of higher methylation of the MDFI promoter with NSCLC may be specific to females, non-smokers and people aged ≤65. D.A. Spandidos 2018-06 2018-04-18 /pmc/articles/PMC5958687/ /pubmed/29805634 http://dx.doi.org/10.3892/ol.2018.8535 Text en Copyright: © Ma et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Ma, Hongying Chen, Xiaoying Hu, Haochang Li, Bin Ying, Xiuru Zhou, Cong Zhong, Jie Zhao, Guofang Duan, Shiwei Hypermethylation of MDFI promoter with NSCLC is specific for females, non-smokers and people younger than 65 |
title | Hypermethylation of MDFI promoter with NSCLC is specific for females, non-smokers and people younger than 65 |
title_full | Hypermethylation of MDFI promoter with NSCLC is specific for females, non-smokers and people younger than 65 |
title_fullStr | Hypermethylation of MDFI promoter with NSCLC is specific for females, non-smokers and people younger than 65 |
title_full_unstemmed | Hypermethylation of MDFI promoter with NSCLC is specific for females, non-smokers and people younger than 65 |
title_short | Hypermethylation of MDFI promoter with NSCLC is specific for females, non-smokers and people younger than 65 |
title_sort | hypermethylation of mdfi promoter with nsclc is specific for females, non-smokers and people younger than 65 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958687/ https://www.ncbi.nlm.nih.gov/pubmed/29805634 http://dx.doi.org/10.3892/ol.2018.8535 |
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