Cargando…
Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion
DEK is a protein ubiquitously expressed in multicellular organisms as well as certain unicellular organisms. It is associated with the regulation of cell proliferation, differentiation, migration, apoptosis, senescence, self-renewal and DNA repairing. In tumor cells it is associated with the carcino...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958825/ https://www.ncbi.nlm.nih.gov/pubmed/29844811 http://dx.doi.org/10.3892/ol.2018.8436 |
_version_ | 1783324297487450112 |
---|---|
author | Zhou, Qian-Cheng Deng, Xue-Feng Yang, Juan Jiang, Hong Qiao, Ming-Xu Liu, Huan-Huan Qian, Zhen Hou, Ling-Ling Hu, Hong-Gang |
author_facet | Zhou, Qian-Cheng Deng, Xue-Feng Yang, Juan Jiang, Hong Qiao, Ming-Xu Liu, Huan-Huan Qian, Zhen Hou, Ling-Ling Hu, Hong-Gang |
author_sort | Zhou, Qian-Cheng |
collection | PubMed |
description | DEK is a protein ubiquitously expressed in multicellular organisms as well as certain unicellular organisms. It is associated with the regulation of cell proliferation, differentiation, migration, apoptosis, senescence, self-renewal and DNA repairing. In tumor cells it is associated with the carcinogenesis process, however there have been few previous studies into the expression of DEK in lung cancer. In the present study the expression level of DEK mRNA and protein was detected in lung cancer tissues and non-cancerous counterparts by performing reverse transcription-quantitative polymerase chain reaction and immunohistochemical staining. It was revealed that the expression of DEK was increased in lung cancer tissues compared with normal tissue. Knock-down and over-expression of DEK in A549 cells were performed to determine the role of DEK in tumor formation. An MTT assay, colony formation assay and Matrigel invasion assay demonstrated that DEK positively regulated cell proliferation and invasion. These results suggest that DEK is highly expressed in lung cancer tissues and positively regulates cell proliferation and invasion. |
format | Online Article Text |
id | pubmed-5958825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-59588252018-05-29 Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion Zhou, Qian-Cheng Deng, Xue-Feng Yang, Juan Jiang, Hong Qiao, Ming-Xu Liu, Huan-Huan Qian, Zhen Hou, Ling-Ling Hu, Hong-Gang Oncol Lett Articles DEK is a protein ubiquitously expressed in multicellular organisms as well as certain unicellular organisms. It is associated with the regulation of cell proliferation, differentiation, migration, apoptosis, senescence, self-renewal and DNA repairing. In tumor cells it is associated with the carcinogenesis process, however there have been few previous studies into the expression of DEK in lung cancer. In the present study the expression level of DEK mRNA and protein was detected in lung cancer tissues and non-cancerous counterparts by performing reverse transcription-quantitative polymerase chain reaction and immunohistochemical staining. It was revealed that the expression of DEK was increased in lung cancer tissues compared with normal tissue. Knock-down and over-expression of DEK in A549 cells were performed to determine the role of DEK in tumor formation. An MTT assay, colony formation assay and Matrigel invasion assay demonstrated that DEK positively regulated cell proliferation and invasion. These results suggest that DEK is highly expressed in lung cancer tissues and positively regulates cell proliferation and invasion. D.A. Spandidos 2018-06 2018-04-05 /pmc/articles/PMC5958825/ /pubmed/29844811 http://dx.doi.org/10.3892/ol.2018.8436 Text en Copyright: © Zhou et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhou, Qian-Cheng Deng, Xue-Feng Yang, Juan Jiang, Hong Qiao, Ming-Xu Liu, Huan-Huan Qian, Zhen Hou, Ling-Ling Hu, Hong-Gang Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion |
title | Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion |
title_full | Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion |
title_fullStr | Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion |
title_full_unstemmed | Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion |
title_short | Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion |
title_sort | oncogene dek is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958825/ https://www.ncbi.nlm.nih.gov/pubmed/29844811 http://dx.doi.org/10.3892/ol.2018.8436 |
work_keys_str_mv | AT zhouqiancheng oncogenedekishighlyexpressedinlungcanceroustissuesandpositivelyregulatescellproliferationaswellasinvasion AT dengxuefeng oncogenedekishighlyexpressedinlungcanceroustissuesandpositivelyregulatescellproliferationaswellasinvasion AT yangjuan oncogenedekishighlyexpressedinlungcanceroustissuesandpositivelyregulatescellproliferationaswellasinvasion AT jianghong oncogenedekishighlyexpressedinlungcanceroustissuesandpositivelyregulatescellproliferationaswellasinvasion AT qiaomingxu oncogenedekishighlyexpressedinlungcanceroustissuesandpositivelyregulatescellproliferationaswellasinvasion AT liuhuanhuan oncogenedekishighlyexpressedinlungcanceroustissuesandpositivelyregulatescellproliferationaswellasinvasion AT qianzhen oncogenedekishighlyexpressedinlungcanceroustissuesandpositivelyregulatescellproliferationaswellasinvasion AT houlingling oncogenedekishighlyexpressedinlungcanceroustissuesandpositivelyregulatescellproliferationaswellasinvasion AT huhonggang oncogenedekishighlyexpressedinlungcanceroustissuesandpositivelyregulatescellproliferationaswellasinvasion |