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Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells
Ewing Sarcoma (ES) is a highly aggressive bone and soft tissue childhood cancer. The development of resistance to chemotherapy is common and remains the main cause of treatment failure. We herein evaluated the expression of genes associated with chemotherapy resistance in ES cell lines. A set of gen...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958871/ https://www.ncbi.nlm.nih.gov/pubmed/29844902 http://dx.doi.org/10.3892/mco.2018.1608 |
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author | Horbach, Leonardo Sinigaglia, Marialva da Silva, Camila Alves Olguins, Danielly Brufatto Gregianin, Lauro José Brunetto, Algemir Lunardi Brunetto, André Tesainer Roesler, Rafael de Farias, Caroline Brunetto |
author_facet | Horbach, Leonardo Sinigaglia, Marialva da Silva, Camila Alves Olguins, Danielly Brufatto Gregianin, Lauro José Brunetto, Algemir Lunardi Brunetto, André Tesainer Roesler, Rafael de Farias, Caroline Brunetto |
author_sort | Horbach, Leonardo |
collection | PubMed |
description | Ewing Sarcoma (ES) is a highly aggressive bone and soft tissue childhood cancer. The development of resistance to chemotherapy is common and remains the main cause of treatment failure. We herein evaluated the expression of genes associated with chemotherapy resistance in ES cell lines. A set of genes (CCAR1, TUBA1A, POLDIP2, SMARCA4 and SMARCB1) was data-mined for resistance against doxorubicin and vincristine, which are the standard drugs used in the treatment of patients with ES. The expression of each gene in SK-ES-1 ES cells was reported before and after exposure to a drug resistance-inducing protocol. There was a significant downregulation of CCAR1 and TUBA1A in doxorubicin-resistant cells, with low expression of TUBA1A in vincristine-resistant cells. By contrast, POLDIP2 was significantly upregulated in cells resistant to either drug, and the expression of the SMARCB1 and SMARCA4 genes was upregulated in doxorubicin-resistant cells. These findings indicate that resistance to specific chemotherapeutic agents was accompanied by differential changes in gene expression in ES tumors. |
format | Online Article Text |
id | pubmed-5958871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-59588712018-05-29 Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells Horbach, Leonardo Sinigaglia, Marialva da Silva, Camila Alves Olguins, Danielly Brufatto Gregianin, Lauro José Brunetto, Algemir Lunardi Brunetto, André Tesainer Roesler, Rafael de Farias, Caroline Brunetto Mol Clin Oncol Articles Ewing Sarcoma (ES) is a highly aggressive bone and soft tissue childhood cancer. The development of resistance to chemotherapy is common and remains the main cause of treatment failure. We herein evaluated the expression of genes associated with chemotherapy resistance in ES cell lines. A set of genes (CCAR1, TUBA1A, POLDIP2, SMARCA4 and SMARCB1) was data-mined for resistance against doxorubicin and vincristine, which are the standard drugs used in the treatment of patients with ES. The expression of each gene in SK-ES-1 ES cells was reported before and after exposure to a drug resistance-inducing protocol. There was a significant downregulation of CCAR1 and TUBA1A in doxorubicin-resistant cells, with low expression of TUBA1A in vincristine-resistant cells. By contrast, POLDIP2 was significantly upregulated in cells resistant to either drug, and the expression of the SMARCB1 and SMARCA4 genes was upregulated in doxorubicin-resistant cells. These findings indicate that resistance to specific chemotherapeutic agents was accompanied by differential changes in gene expression in ES tumors. D.A. Spandidos 2018-06 2018-04-13 /pmc/articles/PMC5958871/ /pubmed/29844902 http://dx.doi.org/10.3892/mco.2018.1608 Text en Copyright: © Horbach et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Horbach, Leonardo Sinigaglia, Marialva da Silva, Camila Alves Olguins, Danielly Brufatto Gregianin, Lauro José Brunetto, Algemir Lunardi Brunetto, André Tesainer Roesler, Rafael de Farias, Caroline Brunetto Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
title | Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
title_full | Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
title_fullStr | Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
title_full_unstemmed | Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
title_short | Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
title_sort | gene expression changes associated with chemotherapy resistance in ewing sarcoma cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958871/ https://www.ncbi.nlm.nih.gov/pubmed/29844902 http://dx.doi.org/10.3892/mco.2018.1608 |
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