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Assessment of EGFR Gene Expression Following Vitrification of 2-cell and Blastocyst Mouse Embryos
BACKGROUND: Exact mechanisms of fetal harm following vitrification are still unknown. This study was conducted to evaluate the cryopreservation impact on the expression of Epidermal Growth Factor Receptor (EGFR) gene in mouse 2-cell and blastocysts. METHODS: To stimulate ovulation in mice, hCG was i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Avicenna Research Institute
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960058/ https://www.ncbi.nlm.nih.gov/pubmed/29849990 |
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author | Gazor, Rouhollah Eskandari, Mozhgan Sharafshah, Alireza Bahadori, Mohammad Hadi Golmohammadi, Mohammad Ghasem Keshavarz, Parvaneh |
author_facet | Gazor, Rouhollah Eskandari, Mozhgan Sharafshah, Alireza Bahadori, Mohammad Hadi Golmohammadi, Mohammad Ghasem Keshavarz, Parvaneh |
author_sort | Gazor, Rouhollah |
collection | PubMed |
description | BACKGROUND: Exact mechanisms of fetal harm following vitrification are still unknown. This study was conducted to evaluate the cryopreservation impact on the expression of Epidermal Growth Factor Receptor (EGFR) gene in mouse 2-cell and blastocysts. METHODS: To stimulate ovulation in mice, hCG was injected, followed by collecting 2-cells and blastocysts after 44–46 and 88–89 hr, respectively. These embryos were divided into two case and control groups. The fresh case group was cryopreserved using cryotop and warmed after 4 mounts. Normal 2-cells were selected based on their morphology and their RNA was extracted. Quantitative expression of EGFR gene in both groups was investigated by applying real time-PCR. RESULTS: The statistical Real-Time (RT)-PCR analyses performed using SPSS revealed that the expression level of EGFR gene was diminished in the case group compared to the control group. CONCLUSION: The current study indicated the negative effect of cryopreservation on expression amount of EGFR gene in 2-cell and blastocyst mouse embryos. |
format | Online Article Text |
id | pubmed-5960058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Avicenna Research Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-59600582018-05-30 Assessment of EGFR Gene Expression Following Vitrification of 2-cell and Blastocyst Mouse Embryos Gazor, Rouhollah Eskandari, Mozhgan Sharafshah, Alireza Bahadori, Mohammad Hadi Golmohammadi, Mohammad Ghasem Keshavarz, Parvaneh Avicenna J Med Biotechnol Short Communication BACKGROUND: Exact mechanisms of fetal harm following vitrification are still unknown. This study was conducted to evaluate the cryopreservation impact on the expression of Epidermal Growth Factor Receptor (EGFR) gene in mouse 2-cell and blastocysts. METHODS: To stimulate ovulation in mice, hCG was injected, followed by collecting 2-cells and blastocysts after 44–46 and 88–89 hr, respectively. These embryos were divided into two case and control groups. The fresh case group was cryopreserved using cryotop and warmed after 4 mounts. Normal 2-cells were selected based on their morphology and their RNA was extracted. Quantitative expression of EGFR gene in both groups was investigated by applying real time-PCR. RESULTS: The statistical Real-Time (RT)-PCR analyses performed using SPSS revealed that the expression level of EGFR gene was diminished in the case group compared to the control group. CONCLUSION: The current study indicated the negative effect of cryopreservation on expression amount of EGFR gene in 2-cell and blastocyst mouse embryos. Avicenna Research Institute 2018 /pmc/articles/PMC5960058/ /pubmed/29849990 Text en Copyright© 2018 Avicenna Research Institute http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Gazor, Rouhollah Eskandari, Mozhgan Sharafshah, Alireza Bahadori, Mohammad Hadi Golmohammadi, Mohammad Ghasem Keshavarz, Parvaneh Assessment of EGFR Gene Expression Following Vitrification of 2-cell and Blastocyst Mouse Embryos |
title | Assessment of EGFR Gene Expression Following Vitrification of 2-cell and Blastocyst Mouse Embryos |
title_full | Assessment of EGFR Gene Expression Following Vitrification of 2-cell and Blastocyst Mouse Embryos |
title_fullStr | Assessment of EGFR Gene Expression Following Vitrification of 2-cell and Blastocyst Mouse Embryos |
title_full_unstemmed | Assessment of EGFR Gene Expression Following Vitrification of 2-cell and Blastocyst Mouse Embryos |
title_short | Assessment of EGFR Gene Expression Following Vitrification of 2-cell and Blastocyst Mouse Embryos |
title_sort | assessment of egfr gene expression following vitrification of 2-cell and blastocyst mouse embryos |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960058/ https://www.ncbi.nlm.nih.gov/pubmed/29849990 |
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