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Isolation and Proliferation of Spermatogonial Cells from Ghezel Sheep

BACKGROUND: Sheep industry has taken steps toward transforming itself into a more efficient and competitive field. There are many varieties of sheep breeds in the world that each of them serves a useful purpose in the economies of different civilizations. Ghezel sheep is one of the Iranian important...

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Autores principales: Qasemi-Panahi, Babak, Movahedin, Mansoureh, Moghaddam, Gholamali, Tajik, Parviz, Koruji, Mortaza, Ashrafi-Helan, Javad, Rafat, Seyed Abbas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960065/
https://www.ncbi.nlm.nih.gov/pubmed/29849985
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author Qasemi-Panahi, Babak
Movahedin, Mansoureh
Moghaddam, Gholamali
Tajik, Parviz
Koruji, Mortaza
Ashrafi-Helan, Javad
Rafat, Seyed Abbas
author_facet Qasemi-Panahi, Babak
Movahedin, Mansoureh
Moghaddam, Gholamali
Tajik, Parviz
Koruji, Mortaza
Ashrafi-Helan, Javad
Rafat, Seyed Abbas
author_sort Qasemi-Panahi, Babak
collection PubMed
description BACKGROUND: Sheep industry has taken steps toward transforming itself into a more efficient and competitive field. There are many varieties of sheep breeds in the world that each of them serves a useful purpose in the economies of different civilizations. Ghezel sheep is one of the Iranian important breeds that are raised for meat, milk and wool. Field of spermatogonial cell technologies provides tools for genetic improvement of sheep herd and multiple opportunities for research. Spermatogonial cells are the only stem cells capable of transmitting genetic information to future generations. METHODS: This study was designed to extend the technique of isolation and in vitro proliferation of spermatogonial cells in Ghezel sheep. RESULTS: Isolated cells were characterized further by using specific markers for type A spermatogonia, including PLZF. Also, sertoli cells were characterized by vimentin which is a specific marker for sertoli cells. After 10 days of co-culture, viability rates of the cells was above 94.7%, but after the freezing process the viability rates were 74 percent. CONCLUSION: In this study, a standard method for isolation and in vitro proliferation of spermatogonial stem cells in Ghezel sheep was developed.
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spelling pubmed-59600652018-05-30 Isolation and Proliferation of Spermatogonial Cells from Ghezel Sheep Qasemi-Panahi, Babak Movahedin, Mansoureh Moghaddam, Gholamali Tajik, Parviz Koruji, Mortaza Ashrafi-Helan, Javad Rafat, Seyed Abbas Avicenna J Med Biotechnol Original Article BACKGROUND: Sheep industry has taken steps toward transforming itself into a more efficient and competitive field. There are many varieties of sheep breeds in the world that each of them serves a useful purpose in the economies of different civilizations. Ghezel sheep is one of the Iranian important breeds that are raised for meat, milk and wool. Field of spermatogonial cell technologies provides tools for genetic improvement of sheep herd and multiple opportunities for research. Spermatogonial cells are the only stem cells capable of transmitting genetic information to future generations. METHODS: This study was designed to extend the technique of isolation and in vitro proliferation of spermatogonial cells in Ghezel sheep. RESULTS: Isolated cells were characterized further by using specific markers for type A spermatogonia, including PLZF. Also, sertoli cells were characterized by vimentin which is a specific marker for sertoli cells. After 10 days of co-culture, viability rates of the cells was above 94.7%, but after the freezing process the viability rates were 74 percent. CONCLUSION: In this study, a standard method for isolation and in vitro proliferation of spermatogonial stem cells in Ghezel sheep was developed. Avicenna Research Institute 2018 /pmc/articles/PMC5960065/ /pubmed/29849985 Text en Copyright© 2018 Avicenna Research Institute http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Qasemi-Panahi, Babak
Movahedin, Mansoureh
Moghaddam, Gholamali
Tajik, Parviz
Koruji, Mortaza
Ashrafi-Helan, Javad
Rafat, Seyed Abbas
Isolation and Proliferation of Spermatogonial Cells from Ghezel Sheep
title Isolation and Proliferation of Spermatogonial Cells from Ghezel Sheep
title_full Isolation and Proliferation of Spermatogonial Cells from Ghezel Sheep
title_fullStr Isolation and Proliferation of Spermatogonial Cells from Ghezel Sheep
title_full_unstemmed Isolation and Proliferation of Spermatogonial Cells from Ghezel Sheep
title_short Isolation and Proliferation of Spermatogonial Cells from Ghezel Sheep
title_sort isolation and proliferation of spermatogonial cells from ghezel sheep
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960065/
https://www.ncbi.nlm.nih.gov/pubmed/29849985
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