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A comparison of cotton and flocked swabs for vaginal self-sample collection

OBJECTIVE: Vaginal self-sampling for human papillomavirus (HPV) testing has recently been proposed to optimize cervical cancer screening coverage. The objective of this study was to compare the performance of self-taken samples using flocked and cotton swabs for HPV detection and cellular retrieval....

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Autores principales: Viviano, Manuela, Willame, Alexia, Cohen, Marie, Benski, Anne-Caroline, Catarino, Rosa, Wuillemin, Christine, Tran, Phuong Lien, Petignat, Patrick, Vassilakos, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960235/
https://www.ncbi.nlm.nih.gov/pubmed/29805267
http://dx.doi.org/10.2147/IJWH.S157897
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author Viviano, Manuela
Willame, Alexia
Cohen, Marie
Benski, Anne-Caroline
Catarino, Rosa
Wuillemin, Christine
Tran, Phuong Lien
Petignat, Patrick
Vassilakos, Pierre
author_facet Viviano, Manuela
Willame, Alexia
Cohen, Marie
Benski, Anne-Caroline
Catarino, Rosa
Wuillemin, Christine
Tran, Phuong Lien
Petignat, Patrick
Vassilakos, Pierre
author_sort Viviano, Manuela
collection PubMed
description OBJECTIVE: Vaginal self-sampling for human papillomavirus (HPV) testing has recently been proposed to optimize cervical cancer screening coverage. The objective of this study was to compare the performance of self-taken samples using flocked and cotton swabs for HPV detection and cellular retrieval. METHODS: We recruited women aged 21–65 years, referred to colposcopy at the Division of Gynecology of the Geneva University Hospitals between May and September 2016. Each participant collected 2 vaginal samples: 1 with a cotton swab and 1 with a flocked swab. A 1:1 randomization determined the order in which the 2 samples were taken. The swabs were introduced into a 20 mL PreservCyt(®) vial. Real-time polymerase chain reaction analysis using the Anyplex™ II HPV HR assay, cytofluorometric analysis and cytological cell counting were performed on each sample. RESULTS: A total of 119 participants were recruited in the study. Their mean ± standard deviation age was 35.1±8.9 years. The HPV prevalence was 29.7% and 38.1% according to the cotton and flocked swab, respectively (p=0.006). The mean number of cells collected per milliliter according to cytofluorometry was 96,726.6 with the cotton swab and 425,544.3 with the flocked swab (p<0.001). The mean number of cells detected at cytological cell count was 13,130.42 using the cotton swab and 17,503.6 using the flocked swab (p<0.001). CONCLUSION: The flocked swab achieved a greater cellular retrieval and showed an improved performance in HPV detection. Further studies are needed to assess the usability and cost-effectiveness of the 2 self-sampling devices.
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spelling pubmed-59602352018-05-25 A comparison of cotton and flocked swabs for vaginal self-sample collection Viviano, Manuela Willame, Alexia Cohen, Marie Benski, Anne-Caroline Catarino, Rosa Wuillemin, Christine Tran, Phuong Lien Petignat, Patrick Vassilakos, Pierre Int J Womens Health Original Research OBJECTIVE: Vaginal self-sampling for human papillomavirus (HPV) testing has recently been proposed to optimize cervical cancer screening coverage. The objective of this study was to compare the performance of self-taken samples using flocked and cotton swabs for HPV detection and cellular retrieval. METHODS: We recruited women aged 21–65 years, referred to colposcopy at the Division of Gynecology of the Geneva University Hospitals between May and September 2016. Each participant collected 2 vaginal samples: 1 with a cotton swab and 1 with a flocked swab. A 1:1 randomization determined the order in which the 2 samples were taken. The swabs were introduced into a 20 mL PreservCyt(®) vial. Real-time polymerase chain reaction analysis using the Anyplex™ II HPV HR assay, cytofluorometric analysis and cytological cell counting were performed on each sample. RESULTS: A total of 119 participants were recruited in the study. Their mean ± standard deviation age was 35.1±8.9 years. The HPV prevalence was 29.7% and 38.1% according to the cotton and flocked swab, respectively (p=0.006). The mean number of cells collected per milliliter according to cytofluorometry was 96,726.6 with the cotton swab and 425,544.3 with the flocked swab (p<0.001). The mean number of cells detected at cytological cell count was 13,130.42 using the cotton swab and 17,503.6 using the flocked swab (p<0.001). CONCLUSION: The flocked swab achieved a greater cellular retrieval and showed an improved performance in HPV detection. Further studies are needed to assess the usability and cost-effectiveness of the 2 self-sampling devices. Dove Medical Press 2018-05-15 /pmc/articles/PMC5960235/ /pubmed/29805267 http://dx.doi.org/10.2147/IJWH.S157897 Text en © 2018 Viviano et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Viviano, Manuela
Willame, Alexia
Cohen, Marie
Benski, Anne-Caroline
Catarino, Rosa
Wuillemin, Christine
Tran, Phuong Lien
Petignat, Patrick
Vassilakos, Pierre
A comparison of cotton and flocked swabs for vaginal self-sample collection
title A comparison of cotton and flocked swabs for vaginal self-sample collection
title_full A comparison of cotton and flocked swabs for vaginal self-sample collection
title_fullStr A comparison of cotton and flocked swabs for vaginal self-sample collection
title_full_unstemmed A comparison of cotton and flocked swabs for vaginal self-sample collection
title_short A comparison of cotton and flocked swabs for vaginal self-sample collection
title_sort comparison of cotton and flocked swabs for vaginal self-sample collection
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960235/
https://www.ncbi.nlm.nih.gov/pubmed/29805267
http://dx.doi.org/10.2147/IJWH.S157897
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