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Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle
Synthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear peptides showed that they failed to induce solid protection in cattle. Dendrimeric peptides displaying two or four cop...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960557/ https://www.ncbi.nlm.nih.gov/pubmed/29854834 http://dx.doi.org/10.1155/2018/3497401 |
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author | Soria, I. Quattrocchi, V. Langellotti, C. Pérez-Filgueira, M. Pega, J. Gnazzo, V. Romera, S. Schammas, J. Bucafusco, D. Di Giacomo, S. de la Torre, Beatriz G. Andreu, D. Sobrino, F. Blanco, E. Zamorano, P. |
author_facet | Soria, I. Quattrocchi, V. Langellotti, C. Pérez-Filgueira, M. Pega, J. Gnazzo, V. Romera, S. Schammas, J. Bucafusco, D. Di Giacomo, S. de la Torre, Beatriz G. Andreu, D. Sobrino, F. Blanco, E. Zamorano, P. |
author_sort | Soria, I. |
collection | PubMed |
description | Synthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear peptides showed that they failed to induce solid protection in cattle. Dendrimeric peptides displaying two or four copies of a peptide corresponding to the B-cell epitope VP1 [136–154] of type O FMDV (O/UKG/11/2001) linked through thioether bonds to a single copy of the T-cell epitope 3A [21–35] (termed B(2)T and B(4)T, resp.) afforded protection in vaccinated pigs. In this work, we show that dendrimeric peptides B(2)T and B(4)T can elicit specific humoral responses in cattle and confer partial protection against the challenge with a heterologous type O virus (O1/Campos/Bra/58). This protective response correlated with the induction of specific T-cells as well as with an anamnestic antibody response upon virus challenge, as shown by the detection of virus-specific antibody-secreting cells (ASC) in lymphoid tissues distal from the inoculation point. |
format | Online Article Text |
id | pubmed-5960557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-59605572018-05-31 Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle Soria, I. Quattrocchi, V. Langellotti, C. Pérez-Filgueira, M. Pega, J. Gnazzo, V. Romera, S. Schammas, J. Bucafusco, D. Di Giacomo, S. de la Torre, Beatriz G. Andreu, D. Sobrino, F. Blanco, E. Zamorano, P. J Immunol Res Research Article Synthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear peptides showed that they failed to induce solid protection in cattle. Dendrimeric peptides displaying two or four copies of a peptide corresponding to the B-cell epitope VP1 [136–154] of type O FMDV (O/UKG/11/2001) linked through thioether bonds to a single copy of the T-cell epitope 3A [21–35] (termed B(2)T and B(4)T, resp.) afforded protection in vaccinated pigs. In this work, we show that dendrimeric peptides B(2)T and B(4)T can elicit specific humoral responses in cattle and confer partial protection against the challenge with a heterologous type O virus (O1/Campos/Bra/58). This protective response correlated with the induction of specific T-cells as well as with an anamnestic antibody response upon virus challenge, as shown by the detection of virus-specific antibody-secreting cells (ASC) in lymphoid tissues distal from the inoculation point. Hindawi 2018-04-18 /pmc/articles/PMC5960557/ /pubmed/29854834 http://dx.doi.org/10.1155/2018/3497401 Text en Copyright © 2018 I. Soria et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Soria, I. Quattrocchi, V. Langellotti, C. Pérez-Filgueira, M. Pega, J. Gnazzo, V. Romera, S. Schammas, J. Bucafusco, D. Di Giacomo, S. de la Torre, Beatriz G. Andreu, D. Sobrino, F. Blanco, E. Zamorano, P. Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
title | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
title_full | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
title_fullStr | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
title_full_unstemmed | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
title_short | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
title_sort | immune response and partial protection against heterologous foot-and-mouth disease virus induced by dendrimer peptides in cattle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960557/ https://www.ncbi.nlm.nih.gov/pubmed/29854834 http://dx.doi.org/10.1155/2018/3497401 |
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