Cargando…

Prevalence of Toxoplasma gondii, Neospora caninum, and Sarcocystis Species DNA in the Heart and Breast Muscles of Rock Pigeons (Columbia livia)

Little is known about the prevalence of protozoan parasites in the muscles of rock pigeons (Columbia livia). The muscles from 54 (heart from 45 and breast from 54) rock pigeons were examined for DNA of Toxoplasma gondii, Neospora caninum, and Sarcocystis species using PCR. Twenty-four were female an...

Descripción completa

Detalles Bibliográficos
Autores principales: Nazir, Muhammad Mudasser, Ayaz, Muhammad Mazhar, Ahmed, Atif Nisar, Maqbool, Azhar, Ashraf, Kamran, Oneeb, Muhammad, Yasin, Ghulam, Subhani, Atta, Ali, Muhammad Asif, Nazir, Noman, Sajid, Muhammad Afzal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960568/
https://www.ncbi.nlm.nih.gov/pubmed/29854422
http://dx.doi.org/10.1155/2018/6264042
_version_ 1783324607926763520
author Nazir, Muhammad Mudasser
Ayaz, Muhammad Mazhar
Ahmed, Atif Nisar
Maqbool, Azhar
Ashraf, Kamran
Oneeb, Muhammad
Yasin, Ghulam
Subhani, Atta
Ali, Muhammad Asif
Nazir, Noman
Sajid, Muhammad Afzal
author_facet Nazir, Muhammad Mudasser
Ayaz, Muhammad Mazhar
Ahmed, Atif Nisar
Maqbool, Azhar
Ashraf, Kamran
Oneeb, Muhammad
Yasin, Ghulam
Subhani, Atta
Ali, Muhammad Asif
Nazir, Noman
Sajid, Muhammad Afzal
author_sort Nazir, Muhammad Mudasser
collection PubMed
description Little is known about the prevalence of protozoan parasites in the muscles of rock pigeons (Columbia livia). The muscles from 54 (heart from 45 and breast from 54) rock pigeons were examined for DNA of Toxoplasma gondii, Neospora caninum, and Sarcocystis species using PCR. Twenty-four were female and 30 were males. The birds were part of flocks of pigeons housed at the tombs of saints in Lahore, Pakistan. Birds that died or were euthanized due to poor health were submitted for necropsy at the Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan, where DNA isolations and PCR were conducted. Nineteen (35.1%) of the birds were positive for T. gondii DNA. Seven males and 12 females were positive. Breast tissue was always infected in T. gondii positive birds, while the heart was infected in 13 (28.8%) of breast positive birds. Five (9.2%) of the pigeons, 2 males and 3 females, were positive for N. caninum. The distribution of N. caninum DNA was more variable in the muscles of pigeons than T. gondii and was found only in the heart of 1 (female), heart and breast muscle of 2 (male), and only the breast muscle of 2 birds (female). One of the 54 rock pigeons (female) was positive for both T. gondii (heart and breast) and N. caninum (heart only). Two of the positive Neospora caninum amplicons were sequenced and had 97% nucleotide identity with N. caninum isolates. Sarcocystis DNA was not found in any bird. The prevalence of T. gondii in rock pigeons and their predation by cats suggest that they may play an unrecognized role in maintaining environmental contamination with T. gondii oocysts by cats. Our study indicates that rock pigeons are intermediate hosts of N. caninum and this information will aid in understanding the epidemiology of N. caninum.
format Online
Article
Text
id pubmed-5960568
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-59605682018-05-31 Prevalence of Toxoplasma gondii, Neospora caninum, and Sarcocystis Species DNA in the Heart and Breast Muscles of Rock Pigeons (Columbia livia) Nazir, Muhammad Mudasser Ayaz, Muhammad Mazhar Ahmed, Atif Nisar Maqbool, Azhar Ashraf, Kamran Oneeb, Muhammad Yasin, Ghulam Subhani, Atta Ali, Muhammad Asif Nazir, Noman Sajid, Muhammad Afzal J Parasitol Res Research Article Little is known about the prevalence of protozoan parasites in the muscles of rock pigeons (Columbia livia). The muscles from 54 (heart from 45 and breast from 54) rock pigeons were examined for DNA of Toxoplasma gondii, Neospora caninum, and Sarcocystis species using PCR. Twenty-four were female and 30 were males. The birds were part of flocks of pigeons housed at the tombs of saints in Lahore, Pakistan. Birds that died or were euthanized due to poor health were submitted for necropsy at the Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan, where DNA isolations and PCR were conducted. Nineteen (35.1%) of the birds were positive for T. gondii DNA. Seven males and 12 females were positive. Breast tissue was always infected in T. gondii positive birds, while the heart was infected in 13 (28.8%) of breast positive birds. Five (9.2%) of the pigeons, 2 males and 3 females, were positive for N. caninum. The distribution of N. caninum DNA was more variable in the muscles of pigeons than T. gondii and was found only in the heart of 1 (female), heart and breast muscle of 2 (male), and only the breast muscle of 2 birds (female). One of the 54 rock pigeons (female) was positive for both T. gondii (heart and breast) and N. caninum (heart only). Two of the positive Neospora caninum amplicons were sequenced and had 97% nucleotide identity with N. caninum isolates. Sarcocystis DNA was not found in any bird. The prevalence of T. gondii in rock pigeons and their predation by cats suggest that they may play an unrecognized role in maintaining environmental contamination with T. gondii oocysts by cats. Our study indicates that rock pigeons are intermediate hosts of N. caninum and this information will aid in understanding the epidemiology of N. caninum. Hindawi 2018-05-06 /pmc/articles/PMC5960568/ /pubmed/29854422 http://dx.doi.org/10.1155/2018/6264042 Text en Copyright © 2018 Muhammad Mudasser Nazir et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nazir, Muhammad Mudasser
Ayaz, Muhammad Mazhar
Ahmed, Atif Nisar
Maqbool, Azhar
Ashraf, Kamran
Oneeb, Muhammad
Yasin, Ghulam
Subhani, Atta
Ali, Muhammad Asif
Nazir, Noman
Sajid, Muhammad Afzal
Prevalence of Toxoplasma gondii, Neospora caninum, and Sarcocystis Species DNA in the Heart and Breast Muscles of Rock Pigeons (Columbia livia)
title Prevalence of Toxoplasma gondii, Neospora caninum, and Sarcocystis Species DNA in the Heart and Breast Muscles of Rock Pigeons (Columbia livia)
title_full Prevalence of Toxoplasma gondii, Neospora caninum, and Sarcocystis Species DNA in the Heart and Breast Muscles of Rock Pigeons (Columbia livia)
title_fullStr Prevalence of Toxoplasma gondii, Neospora caninum, and Sarcocystis Species DNA in the Heart and Breast Muscles of Rock Pigeons (Columbia livia)
title_full_unstemmed Prevalence of Toxoplasma gondii, Neospora caninum, and Sarcocystis Species DNA in the Heart and Breast Muscles of Rock Pigeons (Columbia livia)
title_short Prevalence of Toxoplasma gondii, Neospora caninum, and Sarcocystis Species DNA in the Heart and Breast Muscles of Rock Pigeons (Columbia livia)
title_sort prevalence of toxoplasma gondii, neospora caninum, and sarcocystis species dna in the heart and breast muscles of rock pigeons (columbia livia)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960568/
https://www.ncbi.nlm.nih.gov/pubmed/29854422
http://dx.doi.org/10.1155/2018/6264042
work_keys_str_mv AT nazirmuhammadmudasser prevalenceoftoxoplasmagondiineosporacaninumandsarcocystisspeciesdnaintheheartandbreastmusclesofrockpigeonscolumbialivia
AT ayazmuhammadmazhar prevalenceoftoxoplasmagondiineosporacaninumandsarcocystisspeciesdnaintheheartandbreastmusclesofrockpigeonscolumbialivia
AT ahmedatifnisar prevalenceoftoxoplasmagondiineosporacaninumandsarcocystisspeciesdnaintheheartandbreastmusclesofrockpigeonscolumbialivia
AT maqboolazhar prevalenceoftoxoplasmagondiineosporacaninumandsarcocystisspeciesdnaintheheartandbreastmusclesofrockpigeonscolumbialivia
AT ashrafkamran prevalenceoftoxoplasmagondiineosporacaninumandsarcocystisspeciesdnaintheheartandbreastmusclesofrockpigeonscolumbialivia
AT oneebmuhammad prevalenceoftoxoplasmagondiineosporacaninumandsarcocystisspeciesdnaintheheartandbreastmusclesofrockpigeonscolumbialivia
AT yasinghulam prevalenceoftoxoplasmagondiineosporacaninumandsarcocystisspeciesdnaintheheartandbreastmusclesofrockpigeonscolumbialivia
AT subhaniatta prevalenceoftoxoplasmagondiineosporacaninumandsarcocystisspeciesdnaintheheartandbreastmusclesofrockpigeonscolumbialivia
AT alimuhammadasif prevalenceoftoxoplasmagondiineosporacaninumandsarcocystisspeciesdnaintheheartandbreastmusclesofrockpigeonscolumbialivia
AT nazirnoman prevalenceoftoxoplasmagondiineosporacaninumandsarcocystisspeciesdnaintheheartandbreastmusclesofrockpigeonscolumbialivia
AT sajidmuhammadafzal prevalenceoftoxoplasmagondiineosporacaninumandsarcocystisspeciesdnaintheheartandbreastmusclesofrockpigeonscolumbialivia