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Evaluation of nicotinic receptor of pedunculopontine tegmental nucleus in central cardiovascular regulation in anesthetized rat
OBJECTIVE(S): Cholinergic neurons are important neurons in the Pedunculopontine tegmental nucleus (PPT). In this study, nicotinic receptor of the PPT in central cardiovascular regulation in the anesthetized rat was evaluated. MATERIALS AND METHODS: Saline, acetylcholine (Ach; doses: 90 and 150 nmol)...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960753/ https://www.ncbi.nlm.nih.gov/pubmed/29796220 http://dx.doi.org/10.22038/IJBMS.2018.25616.6319 |
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author | Nikyar, Tahereh Hosseini, Mahmoud Niazmand, Saeed Shafei, Mohammad Naser |
author_facet | Nikyar, Tahereh Hosseini, Mahmoud Niazmand, Saeed Shafei, Mohammad Naser |
author_sort | Nikyar, Tahereh |
collection | PubMed |
description | OBJECTIVE(S): Cholinergic neurons are important neurons in the Pedunculopontine tegmental nucleus (PPT). In this study, nicotinic receptor of the PPT in central cardiovascular regulation in the anesthetized rat was evaluated. MATERIALS AND METHODS: Saline, acetylcholine (Ach; doses: 90 and 150 nmol), hexamethonium (Hexa; doses: 100 and 300 nmol) and higher doses of Hexa (300 nmol) + Ach (150 nmol) microinjected into the PPT. The femoral artery was cannulated and cardiovascular responses were continuously recorded by a power lab system. After injection of drugs, peak changes of mean arterial pressure (∆MAP), systolic blood pressure (∆SBP) and heart rate (∆HR) calculated and compared with saline group. RESULTS: The ∆SBP and ∆MAP significantly decreased by two doses of Ach (P<0.05 to P<0.001) but ∆HR did not change. Two doses 100 (P<0.05) and 300 nmol (P<0.01) of Hexa significantly increased ∆HR but did not alter the ∆MAP or ∆SBP. Co-injection of Hexa + Ach significantly strengthened the ∆HR induced by Hexa alone (P<0.01) but did not affect ∆MAP or ∆SBP. CONCLUSION: These results indicate that nicotinic receptor of the PPT has an inhibitory effect on ∆HR with no significant effect on ∆MAP or ∆SBP. |
format | Online Article Text |
id | pubmed-5960753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-59607532018-05-24 Evaluation of nicotinic receptor of pedunculopontine tegmental nucleus in central cardiovascular regulation in anesthetized rat Nikyar, Tahereh Hosseini, Mahmoud Niazmand, Saeed Shafei, Mohammad Naser Iran J Basic Med Sci Original Article OBJECTIVE(S): Cholinergic neurons are important neurons in the Pedunculopontine tegmental nucleus (PPT). In this study, nicotinic receptor of the PPT in central cardiovascular regulation in the anesthetized rat was evaluated. MATERIALS AND METHODS: Saline, acetylcholine (Ach; doses: 90 and 150 nmol), hexamethonium (Hexa; doses: 100 and 300 nmol) and higher doses of Hexa (300 nmol) + Ach (150 nmol) microinjected into the PPT. The femoral artery was cannulated and cardiovascular responses were continuously recorded by a power lab system. After injection of drugs, peak changes of mean arterial pressure (∆MAP), systolic blood pressure (∆SBP) and heart rate (∆HR) calculated and compared with saline group. RESULTS: The ∆SBP and ∆MAP significantly decreased by two doses of Ach (P<0.05 to P<0.001) but ∆HR did not change. Two doses 100 (P<0.05) and 300 nmol (P<0.01) of Hexa significantly increased ∆HR but did not alter the ∆MAP or ∆SBP. Co-injection of Hexa + Ach significantly strengthened the ∆HR induced by Hexa alone (P<0.01) but did not affect ∆MAP or ∆SBP. CONCLUSION: These results indicate that nicotinic receptor of the PPT has an inhibitory effect on ∆HR with no significant effect on ∆MAP or ∆SBP. Mashhad University of Medical Sciences 2018-04 /pmc/articles/PMC5960753/ /pubmed/29796220 http://dx.doi.org/10.22038/IJBMS.2018.25616.6319 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nikyar, Tahereh Hosseini, Mahmoud Niazmand, Saeed Shafei, Mohammad Naser Evaluation of nicotinic receptor of pedunculopontine tegmental nucleus in central cardiovascular regulation in anesthetized rat |
title | Evaluation of nicotinic receptor of pedunculopontine tegmental nucleus in central cardiovascular regulation in anesthetized rat |
title_full | Evaluation of nicotinic receptor of pedunculopontine tegmental nucleus in central cardiovascular regulation in anesthetized rat |
title_fullStr | Evaluation of nicotinic receptor of pedunculopontine tegmental nucleus in central cardiovascular regulation in anesthetized rat |
title_full_unstemmed | Evaluation of nicotinic receptor of pedunculopontine tegmental nucleus in central cardiovascular regulation in anesthetized rat |
title_short | Evaluation of nicotinic receptor of pedunculopontine tegmental nucleus in central cardiovascular regulation in anesthetized rat |
title_sort | evaluation of nicotinic receptor of pedunculopontine tegmental nucleus in central cardiovascular regulation in anesthetized rat |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960753/ https://www.ncbi.nlm.nih.gov/pubmed/29796220 http://dx.doi.org/10.22038/IJBMS.2018.25616.6319 |
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