Viability test of fish scale collagen (Oshpronemus gouramy) on baby hamster kidney fibroblasts-21 fibroblast cell culture

AIM: This study aims to examine the toxicity of collagen extracted from gouramy fish scales (Oshpronemus gouramy) by evaluating its viability against baby hamster kidney fibroblasts-21. MATERIALS AND METHODS: Collagen was extracted from gouramy fish scales (O. gouramy) with 6% acetic acid. Its resul...

Descripción completa

Detalles Bibliográficos
Autores principales: Prahasanti, Chiquita, Wulandari, Denny Tri, Ulfa, Noer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960791/
https://www.ncbi.nlm.nih.gov/pubmed/29805217
http://dx.doi.org/10.14202/vetworld.2018.506-510
Descripción
Sumario:AIM: This study aims to examine the toxicity of collagen extracted from gouramy fish scales (Oshpronemus gouramy) by evaluating its viability against baby hamster kidney fibroblasts-21. MATERIALS AND METHODS: Collagen was extracted from gouramy fish scales (O. gouramy) with 6% acetic acid. Its results were analyzed using Fourier-transform infrared spectroscopy and freeze-dried technique. Its morphology then was analyzed with scanning electron microscope. Afterward, 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide assay was conducted to compare cells with and without fish scale collagen treatment. RESULTS: Collagen extracted from gouramy fish scales had no influence statistically on cultured fibroblast cells with a statistical significance (2-tailed) value of0.754 (p>00025). CONCLUSION: Collagen extracted from gouramy fish scales has high viability against BHK21 fibroblast cells.

Ejemplares similares