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Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana
The requirements for ascorbate for growth and photosynthesis were assessed under low (LL; 250 µmol m(–2) s(–1)) or high (HL; 1600 µmol m(–2) s(–1)) irradiance in wild-type Arabidopsis thaliana and two ascorbate synthesis mutants (vtc2-1 and vtc2-4) that have 30% wild-type ascorbate levels. The low a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961140/ https://www.ncbi.nlm.nih.gov/pubmed/29726917 http://dx.doi.org/10.1093/jxb/ery170 |
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author | Plumb, William Townsend, Alexandra J Rasool, Brwa Alomrani, Sarah Razak, Nurhayati Karpinska, Barbara Ruban, Alexander V Foyer, Christine H |
author_facet | Plumb, William Townsend, Alexandra J Rasool, Brwa Alomrani, Sarah Razak, Nurhayati Karpinska, Barbara Ruban, Alexander V Foyer, Christine H |
author_sort | Plumb, William |
collection | PubMed |
description | The requirements for ascorbate for growth and photosynthesis were assessed under low (LL; 250 µmol m(–2) s(–1)) or high (HL; 1600 µmol m(–2) s(–1)) irradiance in wild-type Arabidopsis thaliana and two ascorbate synthesis mutants (vtc2-1 and vtc2-4) that have 30% wild-type ascorbate levels. The low ascorbate mutants had the same numbers of leaves but lower rosette area and biomass than the wild type under LL. Wild-type plants experiencing HL had higher leaf ascorbate, anthocyanin, and xanthophyll pigments than under LL. In contrast, leaf ascorbate levels were not increased under HL in the mutant lines. While the degree of oxidation measured using an in vivo redox reporter in the nuclei and cytosol of the leaf epidermal and stomatal cells was similar under both irradiances in all lines, anthocyanin levels were significantly lower in the low ascorbate mutants than in the wild type under HL. Differences in the photosynthetic responses of vtc2-1 and vtc2-4 mutants were observed. Unlike vtc2-1, the vtc2-4 mutants had wild-type zeaxanthin contents. While both low ascorbate mutants had lower levels of non-photochemical quenching of chlorophyll a fluorescence (NPQ) than the wild type under HL, qP(d) values were greater only in vtc2-1 leaves. Ascorbate is therefore essential for growth but not for photoprotection. |
format | Online Article Text |
id | pubmed-5961140 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-59611402018-06-06 Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana Plumb, William Townsend, Alexandra J Rasool, Brwa Alomrani, Sarah Razak, Nurhayati Karpinska, Barbara Ruban, Alexander V Foyer, Christine H J Exp Bot Research Papers The requirements for ascorbate for growth and photosynthesis were assessed under low (LL; 250 µmol m(–2) s(–1)) or high (HL; 1600 µmol m(–2) s(–1)) irradiance in wild-type Arabidopsis thaliana and two ascorbate synthesis mutants (vtc2-1 and vtc2-4) that have 30% wild-type ascorbate levels. The low ascorbate mutants had the same numbers of leaves but lower rosette area and biomass than the wild type under LL. Wild-type plants experiencing HL had higher leaf ascorbate, anthocyanin, and xanthophyll pigments than under LL. In contrast, leaf ascorbate levels were not increased under HL in the mutant lines. While the degree of oxidation measured using an in vivo redox reporter in the nuclei and cytosol of the leaf epidermal and stomatal cells was similar under both irradiances in all lines, anthocyanin levels were significantly lower in the low ascorbate mutants than in the wild type under HL. Differences in the photosynthetic responses of vtc2-1 and vtc2-4 mutants were observed. Unlike vtc2-1, the vtc2-4 mutants had wild-type zeaxanthin contents. While both low ascorbate mutants had lower levels of non-photochemical quenching of chlorophyll a fluorescence (NPQ) than the wild type under HL, qP(d) values were greater only in vtc2-1 leaves. Ascorbate is therefore essential for growth but not for photoprotection. Oxford University Press 2018-05-11 2018-05-03 /pmc/articles/PMC5961140/ /pubmed/29726917 http://dx.doi.org/10.1093/jxb/ery170 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Plumb, William Townsend, Alexandra J Rasool, Brwa Alomrani, Sarah Razak, Nurhayati Karpinska, Barbara Ruban, Alexander V Foyer, Christine H Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana |
title | Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana |
title_full | Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana |
title_fullStr | Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana |
title_full_unstemmed | Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana |
title_short | Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana |
title_sort | ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in arabidopsis thaliana |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961140/ https://www.ncbi.nlm.nih.gov/pubmed/29726917 http://dx.doi.org/10.1093/jxb/ery170 |
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