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Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana

The requirements for ascorbate for growth and photosynthesis were assessed under low (LL; 250 µmol m(–2) s(–1)) or high (HL; 1600 µmol m(–2) s(–1)) irradiance in wild-type Arabidopsis thaliana and two ascorbate synthesis mutants (vtc2-1 and vtc2-4) that have 30% wild-type ascorbate levels. The low a...

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Autores principales: Plumb, William, Townsend, Alexandra J, Rasool, Brwa, Alomrani, Sarah, Razak, Nurhayati, Karpinska, Barbara, Ruban, Alexander V, Foyer, Christine H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961140/
https://www.ncbi.nlm.nih.gov/pubmed/29726917
http://dx.doi.org/10.1093/jxb/ery170
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author Plumb, William
Townsend, Alexandra J
Rasool, Brwa
Alomrani, Sarah
Razak, Nurhayati
Karpinska, Barbara
Ruban, Alexander V
Foyer, Christine H
author_facet Plumb, William
Townsend, Alexandra J
Rasool, Brwa
Alomrani, Sarah
Razak, Nurhayati
Karpinska, Barbara
Ruban, Alexander V
Foyer, Christine H
author_sort Plumb, William
collection PubMed
description The requirements for ascorbate for growth and photosynthesis were assessed under low (LL; 250 µmol m(–2) s(–1)) or high (HL; 1600 µmol m(–2) s(–1)) irradiance in wild-type Arabidopsis thaliana and two ascorbate synthesis mutants (vtc2-1 and vtc2-4) that have 30% wild-type ascorbate levels. The low ascorbate mutants had the same numbers of leaves but lower rosette area and biomass than the wild type under LL. Wild-type plants experiencing HL had higher leaf ascorbate, anthocyanin, and xanthophyll pigments than under LL. In contrast, leaf ascorbate levels were not increased under HL in the mutant lines. While the degree of oxidation measured using an in vivo redox reporter in the nuclei and cytosol of the leaf epidermal and stomatal cells was similar under both irradiances in all lines, anthocyanin levels were significantly lower in the low ascorbate mutants than in the wild type under HL. Differences in the photosynthetic responses of vtc2-1 and vtc2-4 mutants were observed. Unlike vtc2-1, the vtc2-4 mutants had wild-type zeaxanthin contents. While both low ascorbate mutants had lower levels of non-photochemical quenching of chlorophyll a fluorescence (NPQ) than the wild type under HL, qP(d) values were greater only in vtc2-1 leaves. Ascorbate is therefore essential for growth but not for photoprotection.
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spelling pubmed-59611402018-06-06 Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana Plumb, William Townsend, Alexandra J Rasool, Brwa Alomrani, Sarah Razak, Nurhayati Karpinska, Barbara Ruban, Alexander V Foyer, Christine H J Exp Bot Research Papers The requirements for ascorbate for growth and photosynthesis were assessed under low (LL; 250 µmol m(–2) s(–1)) or high (HL; 1600 µmol m(–2) s(–1)) irradiance in wild-type Arabidopsis thaliana and two ascorbate synthesis mutants (vtc2-1 and vtc2-4) that have 30% wild-type ascorbate levels. The low ascorbate mutants had the same numbers of leaves but lower rosette area and biomass than the wild type under LL. Wild-type plants experiencing HL had higher leaf ascorbate, anthocyanin, and xanthophyll pigments than under LL. In contrast, leaf ascorbate levels were not increased under HL in the mutant lines. While the degree of oxidation measured using an in vivo redox reporter in the nuclei and cytosol of the leaf epidermal and stomatal cells was similar under both irradiances in all lines, anthocyanin levels were significantly lower in the low ascorbate mutants than in the wild type under HL. Differences in the photosynthetic responses of vtc2-1 and vtc2-4 mutants were observed. Unlike vtc2-1, the vtc2-4 mutants had wild-type zeaxanthin contents. While both low ascorbate mutants had lower levels of non-photochemical quenching of chlorophyll a fluorescence (NPQ) than the wild type under HL, qP(d) values were greater only in vtc2-1 leaves. Ascorbate is therefore essential for growth but not for photoprotection. Oxford University Press 2018-05-11 2018-05-03 /pmc/articles/PMC5961140/ /pubmed/29726917 http://dx.doi.org/10.1093/jxb/ery170 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Plumb, William
Townsend, Alexandra J
Rasool, Brwa
Alomrani, Sarah
Razak, Nurhayati
Karpinska, Barbara
Ruban, Alexander V
Foyer, Christine H
Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana
title Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana
title_full Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana
title_fullStr Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana
title_full_unstemmed Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana
title_short Ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in Arabidopsis thaliana
title_sort ascorbate-mediated regulation of growth, photoprotection, and photoinhibition in arabidopsis thaliana
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961140/
https://www.ncbi.nlm.nih.gov/pubmed/29726917
http://dx.doi.org/10.1093/jxb/ery170
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