High-throughput construction of multiple cas9 gene variants via assembly of high-depth tiled and sequence-verified oligonucleotides

Selective retrieval of sequence-verified oligonucleotides (oligos) from next-generation sequencing (NGS) flow cells, termed megacloning, promises accurate and reliable gene synthesis. However, gene assembly requires a complete collection of overlapping sense and nonsense oligos, and megacloning does...

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Detalles Bibliográficos
Autores principales: Cho, Namjin, Seo, Han Na, Ryu, Taehoon, Kwon, Euijin, Huh, Sunghoon, Noh, Jinsung, Yeom, Huiran, Hwang, Byungjin, Ha, Heejeong, Lee, Ji Hyun, Kwon, Sunghoon, Bang, Duhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961255/
https://www.ncbi.nlm.nih.gov/pubmed/29529247
http://dx.doi.org/10.1093/nar/gky112

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