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Multiple RPAs make WRN syndrome protein a superhelicase
RPA is known to stimulate the helicase activity of Werner syndrome protein (WRN), but the exact stimulation mechanism is not understood. We use single-molecule FRET and magnetic tweezers to investigate the helicase activity of WRN and its stimulation by RPA. We show that WRN alone is a weak helicase...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961295/ https://www.ncbi.nlm.nih.gov/pubmed/29668972 http://dx.doi.org/10.1093/nar/gky272 |
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author | Lee, Mina Shin, Soochul Uhm, Heesoo Hong, Heesun Kirk, Jaewon Hyun, Kwangbeom Kulikowicz, Tomasz Kim, Jaehoon Ahn, Byungchan Bohr, Vilhelm A Hohng, Sungchul |
author_facet | Lee, Mina Shin, Soochul Uhm, Heesoo Hong, Heesun Kirk, Jaewon Hyun, Kwangbeom Kulikowicz, Tomasz Kim, Jaehoon Ahn, Byungchan Bohr, Vilhelm A Hohng, Sungchul |
author_sort | Lee, Mina |
collection | PubMed |
description | RPA is known to stimulate the helicase activity of Werner syndrome protein (WRN), but the exact stimulation mechanism is not understood. We use single-molecule FRET and magnetic tweezers to investigate the helicase activity of WRN and its stimulation by RPA. We show that WRN alone is a weak helicase which repetitively unwind just a few tens of base pairs, but that binding of multiple RPAs to the enzyme converts WRN into a superhelicase that unidirectionally unwinds double-stranded DNA more than 1 kb. Our study provides a good case in which the activity and biological functions of the enzyme may be fundamentally altered by the binding of cofactors. |
format | Online Article Text |
id | pubmed-5961295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-59612952018-06-06 Multiple RPAs make WRN syndrome protein a superhelicase Lee, Mina Shin, Soochul Uhm, Heesoo Hong, Heesun Kirk, Jaewon Hyun, Kwangbeom Kulikowicz, Tomasz Kim, Jaehoon Ahn, Byungchan Bohr, Vilhelm A Hohng, Sungchul Nucleic Acids Res Nucleic Acid Enzymes RPA is known to stimulate the helicase activity of Werner syndrome protein (WRN), but the exact stimulation mechanism is not understood. We use single-molecule FRET and magnetic tweezers to investigate the helicase activity of WRN and its stimulation by RPA. We show that WRN alone is a weak helicase which repetitively unwind just a few tens of base pairs, but that binding of multiple RPAs to the enzyme converts WRN into a superhelicase that unidirectionally unwinds double-stranded DNA more than 1 kb. Our study provides a good case in which the activity and biological functions of the enzyme may be fundamentally altered by the binding of cofactors. Oxford University Press 2018-05-18 2018-04-14 /pmc/articles/PMC5961295/ /pubmed/29668972 http://dx.doi.org/10.1093/nar/gky272 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Nucleic Acid Enzymes Lee, Mina Shin, Soochul Uhm, Heesoo Hong, Heesun Kirk, Jaewon Hyun, Kwangbeom Kulikowicz, Tomasz Kim, Jaehoon Ahn, Byungchan Bohr, Vilhelm A Hohng, Sungchul Multiple RPAs make WRN syndrome protein a superhelicase |
title | Multiple RPAs make WRN syndrome protein a superhelicase |
title_full | Multiple RPAs make WRN syndrome protein a superhelicase |
title_fullStr | Multiple RPAs make WRN syndrome protein a superhelicase |
title_full_unstemmed | Multiple RPAs make WRN syndrome protein a superhelicase |
title_short | Multiple RPAs make WRN syndrome protein a superhelicase |
title_sort | multiple rpas make wrn syndrome protein a superhelicase |
topic | Nucleic Acid Enzymes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961295/ https://www.ncbi.nlm.nih.gov/pubmed/29668972 http://dx.doi.org/10.1093/nar/gky272 |
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