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Translatome analysis at the egg-to-embryo transition in sea urchin

Early embryogenesis relies on the translational regulation of maternally stored mRNAs. In sea urchin, fertilization triggers a dramatic rise in translation activity, necessary for the onset of cell division. Here, the full spectrum of the mRNAs translated upon fertilization was investigated by polys...

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Autores principales: Chassé, Héloïse, Aubert, Julie, Boulben, Sandrine, Le Corguillé, Gildas, Corre, Erwan, Cormier, Patrick, Morales, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961321/
https://www.ncbi.nlm.nih.gov/pubmed/29660001
http://dx.doi.org/10.1093/nar/gky258
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author Chassé, Héloïse
Aubert, Julie
Boulben, Sandrine
Le Corguillé, Gildas
Corre, Erwan
Cormier, Patrick
Morales, Julia
author_facet Chassé, Héloïse
Aubert, Julie
Boulben, Sandrine
Le Corguillé, Gildas
Corre, Erwan
Cormier, Patrick
Morales, Julia
author_sort Chassé, Héloïse
collection PubMed
description Early embryogenesis relies on the translational regulation of maternally stored mRNAs. In sea urchin, fertilization triggers a dramatic rise in translation activity, necessary for the onset of cell division. Here, the full spectrum of the mRNAs translated upon fertilization was investigated by polysome profiling and sequencing. The translatome of the early sea urchin embryo gave a complete picture of the polysomal recruitment dynamics following fertilization. Our results indicate that only a subset of maternal mRNAs were selectively recruited onto polysomes, with over-represented functional categories in the translated set. The increase in translation upon fertilization depends on the formation of translation initiation complexes following mTOR pathway activation. Surprisingly, mTOR pathway inhibition differentially affected polysomal recruitment of the newly translated mRNAs, which thus appeared either mTOR-dependent or mTOR-independent. Therefore, our data argue for an alternative to the classical cap-dependent model of translation in early development. The identification of the mRNAs translated following fertilization helped assign translational activation events to specific mRNAs. This translatome is the first step to a comprehensive analysis of the molecular mechanisms governing translation upon fertilization and the translational regulatory networks that control the egg-to-embryo transition as well as the early steps of embryogenesis.
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spelling pubmed-59613212018-06-06 Translatome analysis at the egg-to-embryo transition in sea urchin Chassé, Héloïse Aubert, Julie Boulben, Sandrine Le Corguillé, Gildas Corre, Erwan Cormier, Patrick Morales, Julia Nucleic Acids Res Molecular Biology Early embryogenesis relies on the translational regulation of maternally stored mRNAs. In sea urchin, fertilization triggers a dramatic rise in translation activity, necessary for the onset of cell division. Here, the full spectrum of the mRNAs translated upon fertilization was investigated by polysome profiling and sequencing. The translatome of the early sea urchin embryo gave a complete picture of the polysomal recruitment dynamics following fertilization. Our results indicate that only a subset of maternal mRNAs were selectively recruited onto polysomes, with over-represented functional categories in the translated set. The increase in translation upon fertilization depends on the formation of translation initiation complexes following mTOR pathway activation. Surprisingly, mTOR pathway inhibition differentially affected polysomal recruitment of the newly translated mRNAs, which thus appeared either mTOR-dependent or mTOR-independent. Therefore, our data argue for an alternative to the classical cap-dependent model of translation in early development. The identification of the mRNAs translated following fertilization helped assign translational activation events to specific mRNAs. This translatome is the first step to a comprehensive analysis of the molecular mechanisms governing translation upon fertilization and the translational regulatory networks that control the egg-to-embryo transition as well as the early steps of embryogenesis. Oxford University Press 2018-05-18 2018-04-06 /pmc/articles/PMC5961321/ /pubmed/29660001 http://dx.doi.org/10.1093/nar/gky258 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Molecular Biology
Chassé, Héloïse
Aubert, Julie
Boulben, Sandrine
Le Corguillé, Gildas
Corre, Erwan
Cormier, Patrick
Morales, Julia
Translatome analysis at the egg-to-embryo transition in sea urchin
title Translatome analysis at the egg-to-embryo transition in sea urchin
title_full Translatome analysis at the egg-to-embryo transition in sea urchin
title_fullStr Translatome analysis at the egg-to-embryo transition in sea urchin
title_full_unstemmed Translatome analysis at the egg-to-embryo transition in sea urchin
title_short Translatome analysis at the egg-to-embryo transition in sea urchin
title_sort translatome analysis at the egg-to-embryo transition in sea urchin
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961321/
https://www.ncbi.nlm.nih.gov/pubmed/29660001
http://dx.doi.org/10.1093/nar/gky258
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