Fully electronic urine dipstick probe for combinatorial detection of inflammatory biomarkers

AIM: An electrochemical urine dipstick probe biosensor has been demonstrated using molybdenum electrodes on nanoporous polyamide substrate for the quantitative detection of two inflammatory protein biomarkers, CRP and IL-6. MATERIALS & METHODS: The electrode interface was characterized using ζ-p...

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Detalles Bibliográficos
Autores principales: Kamakoti, Vikramshankar, Kinnamon, David, Choi, Kang Hyeok, Jagannath, Badrinath, Prasad, Shalini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961415/
https://www.ncbi.nlm.nih.gov/pubmed/29796304
http://dx.doi.org/10.4155/fsoa-2017-0142
Descripción
Sumario:AIM: An electrochemical urine dipstick probe biosensor has been demonstrated using molybdenum electrodes on nanoporous polyamide substrate for the quantitative detection of two inflammatory protein biomarkers, CRP and IL-6. MATERIALS & METHODS: The electrode interface was characterized using ζ-potential and Fourier transform infrared spectroscopy. Detection of biomarkers was demonstrated by measuring impedance changes associated with the dose concentrations of the two biomarkers. A proof of feasibility of point-of-care implementation of the biosensor was demonstrated using a portable electronics platform. RESULTS & CONCLUSION: Limit of detection of 1 pg/ml was achieved for CRP and IL-6 in human urine and synthetic urine buffers. The developed portable hardware demonstrated close correlation with benchtop equipment results.

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