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Rapid monitoring of the target protein expression with a fluorescent signal based on a dicistronic construct in Escherichia coli
Real-time quantification of recombinant proteins is important in studies on fermentation engineering, cell engineering, etc. Measurement of the expression level of heterologous proteins in bacterial fermentation broth has traditionally relied on time-consuming and labor-intensive procedures, such as...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Berlin Heidelberg
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962521/ https://www.ncbi.nlm.nih.gov/pubmed/29785487 http://dx.doi.org/10.1186/s13568-018-0612-5 |