The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression

SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10–25 µM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci...

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Autores principales: Sen, Onur, Saurin, Adrian T., Higgins, Jonathan M. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962532/
https://www.ncbi.nlm.nih.gov/pubmed/29785044
http://dx.doi.org/10.1038/s41598-018-26307-6
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author Sen, Onur
Saurin, Adrian T.
Higgins, Jonathan M. G.
author_facet Sen, Onur
Saurin, Adrian T.
Higgins, Jonathan M. G.
author_sort Sen, Onur
collection PubMed
description SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10–25 µM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 µM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration.
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spelling pubmed-59625322018-05-24 The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression Sen, Onur Saurin, Adrian T. Higgins, Jonathan M. G. Sci Rep Article SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10–25 µM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 µM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration. Nature Publishing Group UK 2018-05-21 /pmc/articles/PMC5962532/ /pubmed/29785044 http://dx.doi.org/10.1038/s41598-018-26307-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sen, Onur
Saurin, Adrian T.
Higgins, Jonathan M. G.
The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression
title The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression
title_full The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression
title_fullStr The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression
title_full_unstemmed The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression
title_short The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression
title_sort live cell dna stain sir-hoechst induces dna damage responses and impairs cell cycle progression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962532/
https://www.ncbi.nlm.nih.gov/pubmed/29785044
http://dx.doi.org/10.1038/s41598-018-26307-6
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