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The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression
SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10–25 µM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962532/ https://www.ncbi.nlm.nih.gov/pubmed/29785044 http://dx.doi.org/10.1038/s41598-018-26307-6 |