An Oil-Free Picodrop Bioassay Platform for Synthetic Biology

Droplet microfluidics enables massively-parallel analysis of single cells, biomolecules, and chemicals, making it valuable for high-throughput screens. However, many hydrophobic analytes are soluble in carrier oils, preventing their quantitative analysis with the method. We apply Printed Droplet Mic...

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Detalles Bibliográficos
Autores principales: Siltanen, Christian A., Cole, Russell H., Poust, Sean, Chao, Lawrence, Tyerman, Jabus, Kaufmann-Malaga, Benjamin, Ubersax, Jeff, Gartner, Zev J., Abate, Adam R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962535/
https://www.ncbi.nlm.nih.gov/pubmed/29784937
http://dx.doi.org/10.1038/s41598-018-25577-4
Descripción
Sumario:Droplet microfluidics enables massively-parallel analysis of single cells, biomolecules, and chemicals, making it valuable for high-throughput screens. However, many hydrophobic analytes are soluble in carrier oils, preventing their quantitative analysis with the method. We apply Printed Droplet Microfluidics to construct defined reactions with chemicals and cells incubated under air on an open array. The method interfaces with most bioanalytical tools and retains hydrophobic compounds in compartmentalized reactors, allowing their quantitation.

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