An Oil-Free Picodrop Bioassay Platform for Synthetic Biology

Droplet microfluidics enables massively-parallel analysis of single cells, biomolecules, and chemicals, making it valuable for high-throughput screens. However, many hydrophobic analytes are soluble in carrier oils, preventing their quantitative analysis with the method. We apply Printed Droplet Mic...

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Autores principales: Siltanen, Christian A., Cole, Russell H., Poust, Sean, Chao, Lawrence, Tyerman, Jabus, Kaufmann-Malaga, Benjamin, Ubersax, Jeff, Gartner, Zev J., Abate, Adam R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962535/
https://www.ncbi.nlm.nih.gov/pubmed/29784937
http://dx.doi.org/10.1038/s41598-018-25577-4
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author Siltanen, Christian A.
Cole, Russell H.
Poust, Sean
Chao, Lawrence
Tyerman, Jabus
Kaufmann-Malaga, Benjamin
Ubersax, Jeff
Gartner, Zev J.
Abate, Adam R.
author_facet Siltanen, Christian A.
Cole, Russell H.
Poust, Sean
Chao, Lawrence
Tyerman, Jabus
Kaufmann-Malaga, Benjamin
Ubersax, Jeff
Gartner, Zev J.
Abate, Adam R.
author_sort Siltanen, Christian A.
collection PubMed
description Droplet microfluidics enables massively-parallel analysis of single cells, biomolecules, and chemicals, making it valuable for high-throughput screens. However, many hydrophobic analytes are soluble in carrier oils, preventing their quantitative analysis with the method. We apply Printed Droplet Microfluidics to construct defined reactions with chemicals and cells incubated under air on an open array. The method interfaces with most bioanalytical tools and retains hydrophobic compounds in compartmentalized reactors, allowing their quantitation.
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spelling pubmed-59625352018-05-24 An Oil-Free Picodrop Bioassay Platform for Synthetic Biology Siltanen, Christian A. Cole, Russell H. Poust, Sean Chao, Lawrence Tyerman, Jabus Kaufmann-Malaga, Benjamin Ubersax, Jeff Gartner, Zev J. Abate, Adam R. Sci Rep Article Droplet microfluidics enables massively-parallel analysis of single cells, biomolecules, and chemicals, making it valuable for high-throughput screens. However, many hydrophobic analytes are soluble in carrier oils, preventing their quantitative analysis with the method. We apply Printed Droplet Microfluidics to construct defined reactions with chemicals and cells incubated under air on an open array. The method interfaces with most bioanalytical tools and retains hydrophobic compounds in compartmentalized reactors, allowing their quantitation. Nature Publishing Group UK 2018-05-21 /pmc/articles/PMC5962535/ /pubmed/29784937 http://dx.doi.org/10.1038/s41598-018-25577-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Siltanen, Christian A.
Cole, Russell H.
Poust, Sean
Chao, Lawrence
Tyerman, Jabus
Kaufmann-Malaga, Benjamin
Ubersax, Jeff
Gartner, Zev J.
Abate, Adam R.
An Oil-Free Picodrop Bioassay Platform for Synthetic Biology
title An Oil-Free Picodrop Bioassay Platform for Synthetic Biology
title_full An Oil-Free Picodrop Bioassay Platform for Synthetic Biology
title_fullStr An Oil-Free Picodrop Bioassay Platform for Synthetic Biology
title_full_unstemmed An Oil-Free Picodrop Bioassay Platform for Synthetic Biology
title_short An Oil-Free Picodrop Bioassay Platform for Synthetic Biology
title_sort oil-free picodrop bioassay platform for synthetic biology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962535/
https://www.ncbi.nlm.nih.gov/pubmed/29784937
http://dx.doi.org/10.1038/s41598-018-25577-4
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