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Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO
In order to strengthen the current genetically modified organism (GMO) detection system for unauthorized GMO, we have recently developed a new workflow based on DNA walking to amplify unknown sequences surrounding a known DNA region. This DNA walking is performed on transgenic elements, commonly fou...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962636/ https://www.ncbi.nlm.nih.gov/pubmed/29785005 http://dx.doi.org/10.1038/s41598-018-26259-x |
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author | Fraiture, Marie-Alice Saltykova, Assia Hoffman, Stefan Winand, Raf Deforce, Dieter Vanneste, Kevin De Keersmaecker, Sigrid C. J. Roosens, Nancy H. C. |
author_facet | Fraiture, Marie-Alice Saltykova, Assia Hoffman, Stefan Winand, Raf Deforce, Dieter Vanneste, Kevin De Keersmaecker, Sigrid C. J. Roosens, Nancy H. C. |
author_sort | Fraiture, Marie-Alice |
collection | PubMed |
description | In order to strengthen the current genetically modified organism (GMO) detection system for unauthorized GMO, we have recently developed a new workflow based on DNA walking to amplify unknown sequences surrounding a known DNA region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this approach to detect unauthorized GMO via the identification of unique transgene flanking regions and the unnatural associations of elements from the transgenic cassette. In the present study, we investigate the feasibility to integrate the described workflow with the MinION Next-Generation-Sequencing (NGS). The MinION sequencing platform can provide long read-lengths and deal with heterogenic DNA libraries, allowing for rapid and efficient delivery of sequences of interest. In addition, the ability of this NGS platform to characterize unauthorized and unknown GMO without any a priori knowledge has been assessed. |
format | Online Article Text |
id | pubmed-5962636 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59626362018-05-24 Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO Fraiture, Marie-Alice Saltykova, Assia Hoffman, Stefan Winand, Raf Deforce, Dieter Vanneste, Kevin De Keersmaecker, Sigrid C. J. Roosens, Nancy H. C. Sci Rep Article In order to strengthen the current genetically modified organism (GMO) detection system for unauthorized GMO, we have recently developed a new workflow based on DNA walking to amplify unknown sequences surrounding a known DNA region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this approach to detect unauthorized GMO via the identification of unique transgene flanking regions and the unnatural associations of elements from the transgenic cassette. In the present study, we investigate the feasibility to integrate the described workflow with the MinION Next-Generation-Sequencing (NGS). The MinION sequencing platform can provide long read-lengths and deal with heterogenic DNA libraries, allowing for rapid and efficient delivery of sequences of interest. In addition, the ability of this NGS platform to characterize unauthorized and unknown GMO without any a priori knowledge has been assessed. Nature Publishing Group UK 2018-05-21 /pmc/articles/PMC5962636/ /pubmed/29785005 http://dx.doi.org/10.1038/s41598-018-26259-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Fraiture, Marie-Alice Saltykova, Assia Hoffman, Stefan Winand, Raf Deforce, Dieter Vanneste, Kevin De Keersmaecker, Sigrid C. J. Roosens, Nancy H. C. Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO |
title | Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO |
title_full | Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO |
title_fullStr | Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO |
title_full_unstemmed | Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO |
title_short | Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO |
title_sort | nanopore sequencing technology: a new route for the fast detection of unauthorized gmo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962636/ https://www.ncbi.nlm.nih.gov/pubmed/29785005 http://dx.doi.org/10.1038/s41598-018-26259-x |
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