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Aerobic Vitamin B(12) Biosynthesis Is Essential for Pseudomonas aeruginosa Class II Ribonucleotide Reductase Activity During Planktonic and Biofilm Growth

Pseudomonas aeruginosa is a major pathogenic bacterium in chronic infections and is a model organism for studying biofilms. P. aeruginosa is considered an aerobic bacterium, but in the presence of nitrate, it also grows in anaerobic conditions. Oxygen diffusion through the biofilm generates metaboli...

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Autores principales: Crespo, Anna, Blanco-Cabra, Núria, Torrents, Eduard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962746/
https://www.ncbi.nlm.nih.gov/pubmed/29867886
http://dx.doi.org/10.3389/fmicb.2018.00986
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author Crespo, Anna
Blanco-Cabra, Núria
Torrents, Eduard
author_facet Crespo, Anna
Blanco-Cabra, Núria
Torrents, Eduard
author_sort Crespo, Anna
collection PubMed
description Pseudomonas aeruginosa is a major pathogenic bacterium in chronic infections and is a model organism for studying biofilms. P. aeruginosa is considered an aerobic bacterium, but in the presence of nitrate, it also grows in anaerobic conditions. Oxygen diffusion through the biofilm generates metabolic and genetic diversity in P. aeruginosa growth, such as in ribonucleotide reductase activity. These essential enzymes are necessary for DNA synthesis and repair. Oxygen availability determines the activity of the three-ribonucleotide reductase (RNR) classes. Class II and III RNRs are active in the absence of oxygen; however, class II RNRs, which are important in P. aeruginosa biofilm growth, require a vitamin B(12) cofactor for their enzymatic activity. In this work, we elucidated the conditions in which class II RNRs are active due to vitamin B(12) concentration constraints (biosynthesis or environmental availability). We demonstrated that increased vitamin B(12) levels during aerobic, stationary and biofilm growth activate class II RNR activity. We also established that the cobN gene is essentially responsible for B(12) biosynthesis under planktonic and biofilm growth. Our results unravel the mechanisms of dNTP synthesis by P. aeruginosa during biofilm growth, which appear to depend on the bacterial strain (laboratory-type or clinical isolate).
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spelling pubmed-59627462018-06-04 Aerobic Vitamin B(12) Biosynthesis Is Essential for Pseudomonas aeruginosa Class II Ribonucleotide Reductase Activity During Planktonic and Biofilm Growth Crespo, Anna Blanco-Cabra, Núria Torrents, Eduard Front Microbiol Microbiology Pseudomonas aeruginosa is a major pathogenic bacterium in chronic infections and is a model organism for studying biofilms. P. aeruginosa is considered an aerobic bacterium, but in the presence of nitrate, it also grows in anaerobic conditions. Oxygen diffusion through the biofilm generates metabolic and genetic diversity in P. aeruginosa growth, such as in ribonucleotide reductase activity. These essential enzymes are necessary for DNA synthesis and repair. Oxygen availability determines the activity of the three-ribonucleotide reductase (RNR) classes. Class II and III RNRs are active in the absence of oxygen; however, class II RNRs, which are important in P. aeruginosa biofilm growth, require a vitamin B(12) cofactor for their enzymatic activity. In this work, we elucidated the conditions in which class II RNRs are active due to vitamin B(12) concentration constraints (biosynthesis or environmental availability). We demonstrated that increased vitamin B(12) levels during aerobic, stationary and biofilm growth activate class II RNR activity. We also established that the cobN gene is essentially responsible for B(12) biosynthesis under planktonic and biofilm growth. Our results unravel the mechanisms of dNTP synthesis by P. aeruginosa during biofilm growth, which appear to depend on the bacterial strain (laboratory-type or clinical isolate). Frontiers Media S.A. 2018-05-15 /pmc/articles/PMC5962746/ /pubmed/29867886 http://dx.doi.org/10.3389/fmicb.2018.00986 Text en Copyright © 2018 Crespo, Blanco-Cabra and Torrents. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Crespo, Anna
Blanco-Cabra, Núria
Torrents, Eduard
Aerobic Vitamin B(12) Biosynthesis Is Essential for Pseudomonas aeruginosa Class II Ribonucleotide Reductase Activity During Planktonic and Biofilm Growth
title Aerobic Vitamin B(12) Biosynthesis Is Essential for Pseudomonas aeruginosa Class II Ribonucleotide Reductase Activity During Planktonic and Biofilm Growth
title_full Aerobic Vitamin B(12) Biosynthesis Is Essential for Pseudomonas aeruginosa Class II Ribonucleotide Reductase Activity During Planktonic and Biofilm Growth
title_fullStr Aerobic Vitamin B(12) Biosynthesis Is Essential for Pseudomonas aeruginosa Class II Ribonucleotide Reductase Activity During Planktonic and Biofilm Growth
title_full_unstemmed Aerobic Vitamin B(12) Biosynthesis Is Essential for Pseudomonas aeruginosa Class II Ribonucleotide Reductase Activity During Planktonic and Biofilm Growth
title_short Aerobic Vitamin B(12) Biosynthesis Is Essential for Pseudomonas aeruginosa Class II Ribonucleotide Reductase Activity During Planktonic and Biofilm Growth
title_sort aerobic vitamin b(12) biosynthesis is essential for pseudomonas aeruginosa class ii ribonucleotide reductase activity during planktonic and biofilm growth
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962746/
https://www.ncbi.nlm.nih.gov/pubmed/29867886
http://dx.doi.org/10.3389/fmicb.2018.00986
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