Optimizing FRET-FLIM Labeling Conditions to Detect Nuclear Protein Interactions at Native Expression Levels in Living Arabidopsis Roots

Protein complex formation has been extensively studied using Förster resonance energy transfer (FRET) measured by Fluorescence Lifetime Imaging Microscopy (FLIM). However, implementing this technology to detect protein interactions in living multicellular organism at single-cell resolution and under...

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Detalles Bibliográficos
Autores principales: Long, Yuchen, Stahl, Yvonne, Weidtkamp-Peters, Stefanie, Smet, Wouter, Du, Yujuan, Gadella, Theodorus W. J., Goedhart, Joachim, Scheres, Ben, Blilou, Ikram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962846/
https://www.ncbi.nlm.nih.gov/pubmed/29868092
http://dx.doi.org/10.3389/fpls.2018.00639

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