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An Automated Design Framework for Multicellular Recombinase Logic
[Image: see text] Tools to systematically reprogram cellular behavior are crucial to address pressing challenges in manufacturing, environment, or healthcare. Recombinases can very efficiently encode Boolean and history-dependent logic in many species, yet current designs are performed on a case-by-...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962929/ https://www.ncbi.nlm.nih.gov/pubmed/29641183 http://dx.doi.org/10.1021/acssynbio.8b00016 |
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author | Guiziou, Sarah Ulliana, Federico Moreau, Violaine Leclere, Michel Bonnet, Jerome |
author_facet | Guiziou, Sarah Ulliana, Federico Moreau, Violaine Leclere, Michel Bonnet, Jerome |
author_sort | Guiziou, Sarah |
collection | PubMed |
description | [Image: see text] Tools to systematically reprogram cellular behavior are crucial to address pressing challenges in manufacturing, environment, or healthcare. Recombinases can very efficiently encode Boolean and history-dependent logic in many species, yet current designs are performed on a case-by-case basis, limiting their scalability and requiring time-consuming optimization. Here we present an automated workflow for designing recombinase logic devices executing Boolean functions. Our theoretical framework uses a reduced library of computational devices distributed into different cellular subpopulations, which are then composed in various manners to implement all desired logic functions at the multicellular level. Our design platform called CALIN (Composable Asynchronous Logic using Integrase Networks) is broadly accessible via a web server, taking truth tables as inputs and providing corresponding DNA designs and sequences as outputs (available at http://synbio.cbs.cnrs.fr/calin). We anticipate that this automated design workflow will streamline the implementation of Boolean functions in many organisms and for various applications. |
format | Online Article Text |
id | pubmed-5962929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-59629292018-05-23 An Automated Design Framework for Multicellular Recombinase Logic Guiziou, Sarah Ulliana, Federico Moreau, Violaine Leclere, Michel Bonnet, Jerome ACS Synth Biol [Image: see text] Tools to systematically reprogram cellular behavior are crucial to address pressing challenges in manufacturing, environment, or healthcare. Recombinases can very efficiently encode Boolean and history-dependent logic in many species, yet current designs are performed on a case-by-case basis, limiting their scalability and requiring time-consuming optimization. Here we present an automated workflow for designing recombinase logic devices executing Boolean functions. Our theoretical framework uses a reduced library of computational devices distributed into different cellular subpopulations, which are then composed in various manners to implement all desired logic functions at the multicellular level. Our design platform called CALIN (Composable Asynchronous Logic using Integrase Networks) is broadly accessible via a web server, taking truth tables as inputs and providing corresponding DNA designs and sequences as outputs (available at http://synbio.cbs.cnrs.fr/calin). We anticipate that this automated design workflow will streamline the implementation of Boolean functions in many organisms and for various applications. American Chemical Society 2018-04-11 2018-05-18 /pmc/articles/PMC5962929/ /pubmed/29641183 http://dx.doi.org/10.1021/acssynbio.8b00016 Text en Copyright © 2018 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Guiziou, Sarah Ulliana, Federico Moreau, Violaine Leclere, Michel Bonnet, Jerome An Automated Design Framework for Multicellular Recombinase Logic |
title | An Automated Design Framework for Multicellular Recombinase Logic |
title_full | An Automated Design Framework for Multicellular Recombinase Logic |
title_fullStr | An Automated Design Framework for Multicellular Recombinase Logic |
title_full_unstemmed | An Automated Design Framework for Multicellular Recombinase Logic |
title_short | An Automated Design Framework for Multicellular Recombinase Logic |
title_sort | automated design framework for multicellular recombinase logic |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962929/ https://www.ncbi.nlm.nih.gov/pubmed/29641183 http://dx.doi.org/10.1021/acssynbio.8b00016 |
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