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Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction
BACKGROUND: Porcine parvovirus 2 (PPV2) was detected in swine serum without showing any relationship with disease. The emergence of the virus seemed to be a unique event until other genetically highly similar parvoviruses were identified in China and, later in 2012, the presence of the virus was als...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5963090/ https://www.ncbi.nlm.nih.gov/pubmed/29783968 http://dx.doi.org/10.1186/s12917-018-1487-z |
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author | Novosel, Dinko Cadar, Daniel Tuboly, Tamás Jungic, Andreja Stadejek, Tomasz Ait-Ali, Tahar Cságola, Attila |
author_facet | Novosel, Dinko Cadar, Daniel Tuboly, Tamás Jungic, Andreja Stadejek, Tomasz Ait-Ali, Tahar Cságola, Attila |
author_sort | Novosel, Dinko |
collection | PubMed |
description | BACKGROUND: Porcine parvovirus 2 (PPV2) was detected in swine serum without showing any relationship with disease. The emergence of the virus seemed to be a unique event until other genetically highly similar parvoviruses were identified in China and, later in 2012, the presence of the virus was also described in Europe. PPV2 is widely distributed in pig populations where it is suspected to be involved in respiratory conditions, based on its frequent detection in lung samples. In order to investigate the potential pathogenic involvement of PPV2, 60 dead pigs were examined from two farms. They were necropsied and tested for PPV2 and PCV2 (Porcine circovirus type 2) by PCR; by Brown and Brenn (B&B) staining for bacteria; by immunohistochemistry (IHC) to detect CD3, Swine leukocyte antigen class II DQ (SLAIIDQ), lysozyme, porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza (SIV), Mycoplasma hyopneumoniae (Mhyo); and by in situ hybridization (ISH) to detect ssDNA and dsDNA of PCV2. PPV2 positive samples were subjected to in situ polymerase chain reaction (IS-PCR) including double staining method to detect PPV2 and host cell markers. To calculate statistical difference we used GENMOD or LOGISTIC procedures in Statistical Analysis System (SAS®). RESULTS: We found that the PPV2 was localized mostly in lymphocytes in lungs, lymph nodes and liver. Neither CD3 antigen nor lysozyme was expressed by these infected cells. In contrast, low levels of SLAIIDQ were expressed by infected cells, suggesting that PPV2 may have a specific tropism for immature B lymphocytes and/or NK lymphocytes though possibly not T lymphocytes. CONCLUSION: The overall conclusion of this study indicates that PPV2 may contribute to the pathogenesis of pneumonia. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-018-1487-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5963090 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-59630902018-06-25 Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction Novosel, Dinko Cadar, Daniel Tuboly, Tamás Jungic, Andreja Stadejek, Tomasz Ait-Ali, Tahar Cságola, Attila BMC Vet Res Research Article BACKGROUND: Porcine parvovirus 2 (PPV2) was detected in swine serum without showing any relationship with disease. The emergence of the virus seemed to be a unique event until other genetically highly similar parvoviruses were identified in China and, later in 2012, the presence of the virus was also described in Europe. PPV2 is widely distributed in pig populations where it is suspected to be involved in respiratory conditions, based on its frequent detection in lung samples. In order to investigate the potential pathogenic involvement of PPV2, 60 dead pigs were examined from two farms. They were necropsied and tested for PPV2 and PCV2 (Porcine circovirus type 2) by PCR; by Brown and Brenn (B&B) staining for bacteria; by immunohistochemistry (IHC) to detect CD3, Swine leukocyte antigen class II DQ (SLAIIDQ), lysozyme, porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza (SIV), Mycoplasma hyopneumoniae (Mhyo); and by in situ hybridization (ISH) to detect ssDNA and dsDNA of PCV2. PPV2 positive samples were subjected to in situ polymerase chain reaction (IS-PCR) including double staining method to detect PPV2 and host cell markers. To calculate statistical difference we used GENMOD or LOGISTIC procedures in Statistical Analysis System (SAS®). RESULTS: We found that the PPV2 was localized mostly in lymphocytes in lungs, lymph nodes and liver. Neither CD3 antigen nor lysozyme was expressed by these infected cells. In contrast, low levels of SLAIIDQ were expressed by infected cells, suggesting that PPV2 may have a specific tropism for immature B lymphocytes and/or NK lymphocytes though possibly not T lymphocytes. CONCLUSION: The overall conclusion of this study indicates that PPV2 may contribute to the pathogenesis of pneumonia. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-018-1487-z) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-21 /pmc/articles/PMC5963090/ /pubmed/29783968 http://dx.doi.org/10.1186/s12917-018-1487-z Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Novosel, Dinko Cadar, Daniel Tuboly, Tamás Jungic, Andreja Stadejek, Tomasz Ait-Ali, Tahar Cságola, Attila Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction |
title | Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction |
title_full | Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction |
title_fullStr | Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction |
title_full_unstemmed | Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction |
title_short | Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction |
title_sort | investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5963090/ https://www.ncbi.nlm.nih.gov/pubmed/29783968 http://dx.doi.org/10.1186/s12917-018-1487-z |
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