Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis

OBJECTIVE: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australi...

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Autores principales: Beissbarth, Jemima, Binks, Michael J., Marsh, Robyn L., Chang, Anne B., Leach, Amanda J., Smith-Vaughan, Heidi C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5963172/
https://www.ncbi.nlm.nih.gov/pubmed/29784027
http://dx.doi.org/10.1186/s13104-018-3429-z
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author Beissbarth, Jemima
Binks, Michael J.
Marsh, Robyn L.
Chang, Anne B.
Leach, Amanda J.
Smith-Vaughan, Heidi C.
author_facet Beissbarth, Jemima
Binks, Michael J.
Marsh, Robyn L.
Chang, Anne B.
Leach, Amanda J.
Smith-Vaughan, Heidi C.
author_sort Beissbarth, Jemima
collection PubMed
description OBJECTIVE: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australia. RESULTS: Among respiratory specimens collected in studies between 2010 and 2013, retrospective PCR analysis found that routine culture misidentified H. haemolyticus as NTHi in 0.3% (3/879) of nasal specimens, 25% (14/55) of bronchoalveolar lavage and 40% (12/30) of throat specimens. Therefore, in this population, PCR-based NTHi diagnostics are indicated for throat and bronchoalveolar specimens, but not for nasal specimens.
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spelling pubmed-59631722018-05-24 Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis Beissbarth, Jemima Binks, Michael J. Marsh, Robyn L. Chang, Anne B. Leach, Amanda J. Smith-Vaughan, Heidi C. BMC Res Notes Research Note OBJECTIVE: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australia. RESULTS: Among respiratory specimens collected in studies between 2010 and 2013, retrospective PCR analysis found that routine culture misidentified H. haemolyticus as NTHi in 0.3% (3/879) of nasal specimens, 25% (14/55) of bronchoalveolar lavage and 40% (12/30) of throat specimens. Therefore, in this population, PCR-based NTHi diagnostics are indicated for throat and bronchoalveolar specimens, but not for nasal specimens. BioMed Central 2018-05-21 /pmc/articles/PMC5963172/ /pubmed/29784027 http://dx.doi.org/10.1186/s13104-018-3429-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Beissbarth, Jemima
Binks, Michael J.
Marsh, Robyn L.
Chang, Anne B.
Leach, Amanda J.
Smith-Vaughan, Heidi C.
Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis
title Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis
title_full Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis
title_fullStr Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis
title_full_unstemmed Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis
title_short Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis
title_sort recommendations for application of haemophilus influenzae pcr diagnostics to respiratory specimens for children living in northern australia: a retrospective re-analysis
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5963172/
https://www.ncbi.nlm.nih.gov/pubmed/29784027
http://dx.doi.org/10.1186/s13104-018-3429-z
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