Phenolic Profile, Antiradical and Antitumour Evaluation of Raspberries Pomace Extract from Serbia

Raspberry waste, obtained from two cultivars, Meeker (ERM) and Willamette (ERW) was subjected to evaluation antioxidants and antitumour activities as a potential source of phenolics. Some phenolic acids and flavonoids were identified and quantified by HPLC. Antioxidant activity was tested by measuring ability to scavenge DPPH(•) and (•)OH by ESR spectroscopy. IC(50)(DPPH•) varied from 0.67 for ERM to 0.54 mg/mL for ERW, while IC(50)(•OH) values varied from 3.73 for ERM to 1.23 mg/mL for ERW. Cytotoxic activity was investigated using in vivo model of Ehrlich Ascite carcinoma cells (EAC) in mice. Pretreatment with extracts exhibited potent cytotoxic activity against EAC cells (up to 60%) and both extracts inhibited the tumour growth. Activity of xanthine oxidase (XOD) was extremely increased in pretreated animals, while the activity of enzyme complex glutathione reductase (GR) and glutathione peroxidase (GSHPx) was significantly increased. This study suggests that raspberry pomace could be used as nutraceutic resource and functional food ingredient.