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Novel transgenic pigs with enhanced growth and reduced environmental impact
In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing t...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5963925/ https://www.ncbi.nlm.nih.gov/pubmed/29784082 http://dx.doi.org/10.7554/eLife.34286 |
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author | Zhang, Xianwei Li, Zicong Yang, Huaqiang Liu, Dewu Cai, Gengyuan Li, Guoling Mo, Jianxin Wang, Dehua Zhong, Cuili Wang, Haoqiang Sun, Yue Shi, Junsong Zheng, Enqin Meng, Fanming Zhang, Mao He, Xiaoyan Zhou, Rong Zhang, Jian Huang, Miaorong Zhang, Ran Li, Ning Fan, Mingzhe Yang, Jinzeng Wu, Zhenfang |
author_facet | Zhang, Xianwei Li, Zicong Yang, Huaqiang Liu, Dewu Cai, Gengyuan Li, Guoling Mo, Jianxin Wang, Dehua Zhong, Cuili Wang, Haoqiang Sun, Yue Shi, Junsong Zheng, Enqin Meng, Fanming Zhang, Mao He, Xiaoyan Zhou, Rong Zhang, Jian Huang, Miaorong Zhang, Ran Li, Ning Fan, Mingzhe Yang, Jinzeng Wu, Zhenfang |
author_sort | Zhang, Xianwei |
collection | PubMed |
description | In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact. |
format | Online Article Text |
id | pubmed-5963925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59639252018-05-24 Novel transgenic pigs with enhanced growth and reduced environmental impact Zhang, Xianwei Li, Zicong Yang, Huaqiang Liu, Dewu Cai, Gengyuan Li, Guoling Mo, Jianxin Wang, Dehua Zhong, Cuili Wang, Haoqiang Sun, Yue Shi, Junsong Zheng, Enqin Meng, Fanming Zhang, Mao He, Xiaoyan Zhou, Rong Zhang, Jian Huang, Miaorong Zhang, Ran Li, Ning Fan, Mingzhe Yang, Jinzeng Wu, Zhenfang eLife Biochemistry and Chemical Biology In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact. eLife Sciences Publications, Ltd 2018-05-22 /pmc/articles/PMC5963925/ /pubmed/29784082 http://dx.doi.org/10.7554/eLife.34286 Text en © 2018, Zhang et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology Zhang, Xianwei Li, Zicong Yang, Huaqiang Liu, Dewu Cai, Gengyuan Li, Guoling Mo, Jianxin Wang, Dehua Zhong, Cuili Wang, Haoqiang Sun, Yue Shi, Junsong Zheng, Enqin Meng, Fanming Zhang, Mao He, Xiaoyan Zhou, Rong Zhang, Jian Huang, Miaorong Zhang, Ran Li, Ning Fan, Mingzhe Yang, Jinzeng Wu, Zhenfang Novel transgenic pigs with enhanced growth and reduced environmental impact |
title | Novel transgenic pigs with enhanced growth and reduced environmental impact |
title_full | Novel transgenic pigs with enhanced growth and reduced environmental impact |
title_fullStr | Novel transgenic pigs with enhanced growth and reduced environmental impact |
title_full_unstemmed | Novel transgenic pigs with enhanced growth and reduced environmental impact |
title_short | Novel transgenic pigs with enhanced growth and reduced environmental impact |
title_sort | novel transgenic pigs with enhanced growth and reduced environmental impact |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5963925/ https://www.ncbi.nlm.nih.gov/pubmed/29784082 http://dx.doi.org/10.7554/eLife.34286 |
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