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Molecular Screening of Small Biopsy Samples Using Next-Generation Sequencing in Korean Patients with Advanced Non-small Cell Lung Cancer: Korean Lung Cancer Consortium (KLCC-13-01)

BACKGROUND: Non-small cell lung cancer (NSCLC) is a common type of cancer with poor prognosis. As individual cancers exhibit unique mutation patterns, identifying and characterizing gene mutations in NSCLC might help predict patient outcomes and guide treatment. The aim of this study was to evaluate...

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Autores principales: Ku, Bo Mi, Heo, Mi Hwa, Kim, Joo-Hang, Cho, Byoung Chul, Cho, Eun Kyung, Min, Young Joo, Lee, Ki Hyeong, Sun, Jong-Mu, Lee, Se-Hoon, Ahn, Jin Seok, Park, Keunchil, Kim, Tae Jung, Lee, Ho Yun, Kim, Hojoong, Lee, Kyung-Jong, Ahn, Myung-Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Pathologists and the Korean Society for Cytopathology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964289/
https://www.ncbi.nlm.nih.gov/pubmed/29575851
http://dx.doi.org/10.4132/jptm.2018.03.12
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author Ku, Bo Mi
Heo, Mi Hwa
Kim, Joo-Hang
Cho, Byoung Chul
Cho, Eun Kyung
Min, Young Joo
Lee, Ki Hyeong
Sun, Jong-Mu
Lee, Se-Hoon
Ahn, Jin Seok
Park, Keunchil
Kim, Tae Jung
Lee, Ho Yun
Kim, Hojoong
Lee, Kyung-Jong
Ahn, Myung-Ju
author_facet Ku, Bo Mi
Heo, Mi Hwa
Kim, Joo-Hang
Cho, Byoung Chul
Cho, Eun Kyung
Min, Young Joo
Lee, Ki Hyeong
Sun, Jong-Mu
Lee, Se-Hoon
Ahn, Jin Seok
Park, Keunchil
Kim, Tae Jung
Lee, Ho Yun
Kim, Hojoong
Lee, Kyung-Jong
Ahn, Myung-Ju
author_sort Ku, Bo Mi
collection PubMed
description BACKGROUND: Non-small cell lung cancer (NSCLC) is a common type of cancer with poor prognosis. As individual cancers exhibit unique mutation patterns, identifying and characterizing gene mutations in NSCLC might help predict patient outcomes and guide treatment. The aim of this study was to evaluate the clinical adequacy of molecular testing using next-generation sequencing (NGS) for small biopsy samples and characterize the mutational landscape of Korean patients with advanced NSCLC. METHODS: DNA was extracted from small biopsy samples of 162 patients with advanced NSCLC. Targeted NGS of genomic alterations was conducted using Ion AmpliSeq Cancer Hotspot Panel v2. RESULTS: The median age of patients was 64 years (range, 32 to 83 years) and the majority had stage IV NSCLC at the time of cancer diagnosis (90%). Among the 162 patients, 161 patients (99.4%) had novel or hotspot mutations (range, 1 to 21 mutated genes). Mutations were found in 41 genes. Three of the most frequently mutated genes were TP53 (151, 93.2%), KDR (104, 64.2%), and epidermal growth factor receptor (EGFR; 69, 42.6%). We also observed coexistence of EGFR and other oncogene (such as KRAS, PIC3CA, PTEN, and STK11) mutations. Given that 69.6% (48/69) of EGFR mutant patients were treated with EGFR tyrosine kinase inhibitors, EGFR mutant status had higher prognostic ability in this study. CONCLUSIONS: These results suggest that targeted NGS using small biopsy samples is feasible and allows for the detection of both common and rare mutations in NSCLC.
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spelling pubmed-59642892018-05-31 Molecular Screening of Small Biopsy Samples Using Next-Generation Sequencing in Korean Patients with Advanced Non-small Cell Lung Cancer: Korean Lung Cancer Consortium (KLCC-13-01) Ku, Bo Mi Heo, Mi Hwa Kim, Joo-Hang Cho, Byoung Chul Cho, Eun Kyung Min, Young Joo Lee, Ki Hyeong Sun, Jong-Mu Lee, Se-Hoon Ahn, Jin Seok Park, Keunchil Kim, Tae Jung Lee, Ho Yun Kim, Hojoong Lee, Kyung-Jong Ahn, Myung-Ju J Pathol Transl Med Original Article BACKGROUND: Non-small cell lung cancer (NSCLC) is a common type of cancer with poor prognosis. As individual cancers exhibit unique mutation patterns, identifying and characterizing gene mutations in NSCLC might help predict patient outcomes and guide treatment. The aim of this study was to evaluate the clinical adequacy of molecular testing using next-generation sequencing (NGS) for small biopsy samples and characterize the mutational landscape of Korean patients with advanced NSCLC. METHODS: DNA was extracted from small biopsy samples of 162 patients with advanced NSCLC. Targeted NGS of genomic alterations was conducted using Ion AmpliSeq Cancer Hotspot Panel v2. RESULTS: The median age of patients was 64 years (range, 32 to 83 years) and the majority had stage IV NSCLC at the time of cancer diagnosis (90%). Among the 162 patients, 161 patients (99.4%) had novel or hotspot mutations (range, 1 to 21 mutated genes). Mutations were found in 41 genes. Three of the most frequently mutated genes were TP53 (151, 93.2%), KDR (104, 64.2%), and epidermal growth factor receptor (EGFR; 69, 42.6%). We also observed coexistence of EGFR and other oncogene (such as KRAS, PIC3CA, PTEN, and STK11) mutations. Given that 69.6% (48/69) of EGFR mutant patients were treated with EGFR tyrosine kinase inhibitors, EGFR mutant status had higher prognostic ability in this study. CONCLUSIONS: These results suggest that targeted NGS using small biopsy samples is feasible and allows for the detection of both common and rare mutations in NSCLC. The Korean Society of Pathologists and the Korean Society for Cytopathology 2018-05 2018-03-26 /pmc/articles/PMC5964289/ /pubmed/29575851 http://dx.doi.org/10.4132/jptm.2018.03.12 Text en © 2018 The Korean Society of Pathologists/The Korean Society for Cytopathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ku, Bo Mi
Heo, Mi Hwa
Kim, Joo-Hang
Cho, Byoung Chul
Cho, Eun Kyung
Min, Young Joo
Lee, Ki Hyeong
Sun, Jong-Mu
Lee, Se-Hoon
Ahn, Jin Seok
Park, Keunchil
Kim, Tae Jung
Lee, Ho Yun
Kim, Hojoong
Lee, Kyung-Jong
Ahn, Myung-Ju
Molecular Screening of Small Biopsy Samples Using Next-Generation Sequencing in Korean Patients with Advanced Non-small Cell Lung Cancer: Korean Lung Cancer Consortium (KLCC-13-01)
title Molecular Screening of Small Biopsy Samples Using Next-Generation Sequencing in Korean Patients with Advanced Non-small Cell Lung Cancer: Korean Lung Cancer Consortium (KLCC-13-01)
title_full Molecular Screening of Small Biopsy Samples Using Next-Generation Sequencing in Korean Patients with Advanced Non-small Cell Lung Cancer: Korean Lung Cancer Consortium (KLCC-13-01)
title_fullStr Molecular Screening of Small Biopsy Samples Using Next-Generation Sequencing in Korean Patients with Advanced Non-small Cell Lung Cancer: Korean Lung Cancer Consortium (KLCC-13-01)
title_full_unstemmed Molecular Screening of Small Biopsy Samples Using Next-Generation Sequencing in Korean Patients with Advanced Non-small Cell Lung Cancer: Korean Lung Cancer Consortium (KLCC-13-01)
title_short Molecular Screening of Small Biopsy Samples Using Next-Generation Sequencing in Korean Patients with Advanced Non-small Cell Lung Cancer: Korean Lung Cancer Consortium (KLCC-13-01)
title_sort molecular screening of small biopsy samples using next-generation sequencing in korean patients with advanced non-small cell lung cancer: korean lung cancer consortium (klcc-13-01)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964289/
https://www.ncbi.nlm.nih.gov/pubmed/29575851
http://dx.doi.org/10.4132/jptm.2018.03.12
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