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CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response
Dendritic cells (DCs) are crucial for the balance between immune response and tolerance, but the molecular mechanism regulating development, differentiation, and homeostasis are poorly understood. The transcriptional activator CREB is involved in regulating different cells of the innate and adaptive...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964551/ https://www.ncbi.nlm.nih.gov/pubmed/29854847 http://dx.doi.org/10.1155/2018/8947230 |
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author | Ohl, Kim Schippers, Anastasia Tenbrock, Klaus |
author_facet | Ohl, Kim Schippers, Anastasia Tenbrock, Klaus |
author_sort | Ohl, Kim |
collection | PubMed |
description | Dendritic cells (DCs) are crucial for the balance between immune response and tolerance, but the molecular mechanism regulating development, differentiation, and homeostasis are poorly understood. The transcriptional activator CREB is involved in regulating different cells of the innate and adaptive immune system and is a transcriptional regulator of development, survival, activation, or proliferation in macrophages, dendritic cells, B cells, and T cells. To directly examine the role of CREB in the regulation of DCs, the CREB gene was targeted for deletion with a CD11c-cre transgene. The deletion of CREB in CD11c(+) cells did not involve any developmental or systemic defects within DC populations. However, CREB deficiency in CD11c(+) cells reduced germinal center (GC) B cells in steady state, and immunization with NP-CGG resulted in a reduced formation of GCs, paralleled by the reduced production of IgGs in sera of immunized mice. In conclusion, we demonstrate that CREB expression in CD11c(+) cells enhances germinal center responses, most likely by altering DC function, which might have implications for autoimmune diseases that are associated with dysregulated GC responses. |
format | Online Article Text |
id | pubmed-5964551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-59645512018-05-31 CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response Ohl, Kim Schippers, Anastasia Tenbrock, Klaus J Immunol Res Research Article Dendritic cells (DCs) are crucial for the balance between immune response and tolerance, but the molecular mechanism regulating development, differentiation, and homeostasis are poorly understood. The transcriptional activator CREB is involved in regulating different cells of the innate and adaptive immune system and is a transcriptional regulator of development, survival, activation, or proliferation in macrophages, dendritic cells, B cells, and T cells. To directly examine the role of CREB in the regulation of DCs, the CREB gene was targeted for deletion with a CD11c-cre transgene. The deletion of CREB in CD11c(+) cells did not involve any developmental or systemic defects within DC populations. However, CREB deficiency in CD11c(+) cells reduced germinal center (GC) B cells in steady state, and immunization with NP-CGG resulted in a reduced formation of GCs, paralleled by the reduced production of IgGs in sera of immunized mice. In conclusion, we demonstrate that CREB expression in CD11c(+) cells enhances germinal center responses, most likely by altering DC function, which might have implications for autoimmune diseases that are associated with dysregulated GC responses. Hindawi 2018-05-07 /pmc/articles/PMC5964551/ /pubmed/29854847 http://dx.doi.org/10.1155/2018/8947230 Text en Copyright © 2018 Kim Ohl et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ohl, Kim Schippers, Anastasia Tenbrock, Klaus CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response |
title | CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response |
title_full | CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response |
title_fullStr | CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response |
title_full_unstemmed | CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response |
title_short | CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response |
title_sort | cd11c-specific deletion reveals creb as a critical regulator of dc function during the germinal center response |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964551/ https://www.ncbi.nlm.nih.gov/pubmed/29854847 http://dx.doi.org/10.1155/2018/8947230 |
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