Cargando…

GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

BACKGROUND: With the widespread use of rifampicin and isoniazid, bacterial resistance has become a growing problem. Additionally, the lack of relevant baseline information for the frequency of drug-resistant tuberculosis (TB) gene mutations is a critical issue, and the incidence of this infection in...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Ming-Jin, Ren, Wen-Zhi, Sun, Xue-Juan, Liu, Yang, Liu, Ke-Wei, Ji, Zhong-Hao, Gao, Wei, Yuan, Bao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964880/
https://www.ncbi.nlm.nih.gov/pubmed/29788948
http://dx.doi.org/10.1186/s12879-018-3131-8
_version_ 1783325260724043776
author Zhang, Ming-Jin
Ren, Wen-Zhi
Sun, Xue-Juan
Liu, Yang
Liu, Ke-Wei
Ji, Zhong-Hao
Gao, Wei
Yuan, Bao
author_facet Zhang, Ming-Jin
Ren, Wen-Zhi
Sun, Xue-Juan
Liu, Yang
Liu, Ke-Wei
Ji, Zhong-Hao
Gao, Wei
Yuan, Bao
author_sort Zhang, Ming-Jin
collection PubMed
description BACKGROUND: With the widespread use of rifampicin and isoniazid, bacterial resistance has become a growing problem. Additionally, the lack of relevant baseline information for the frequency of drug-resistant tuberculosis (TB) gene mutations is a critical issue, and the incidence of this infection in the city of Changchun has not investigated to date. However, compared with the slow traditional methods of drug susceptibility testing, recently developed detection methods, such as rifampicin and isoniazid resistance-related gene chip techniques, allow for rapid, easy detection and simultaneous testing for mutation frequency and drug resistance. METHODS: In this study, the rifampicin and isoniazid resistance-related gene mutation chip method was employed for an epidemiological investigation. To assess the gene mutation characteristics of drug-resistant TB and evaluate the chip method, we tested 2143 clinical specimens from patients from the infectious diseases hospital of Changchun city from January to December 2016. The drug sensitivity test method was used as the reference standard. RESULTS: The following mutation frequencies of sites in the rifampicin resistance gene rpoB were found: Ser531Leu (52.6%), His526Tyr (12.3%), and Leu511Pro (8.8%). The multidrug-resistance (MDR)-TB mutation frequency was 34.7% for rpoB Ser531Leu and katG Ser315Thr, 26.4% for rpoB Ser531Leu and inhA promoter − 15 (C → T), and 10.7% for rpoB His526Tyr and katG Ser315Thr. In addition, drug susceptibility testing served as a reference standard. In previously treated clinical cases, the sensitivity and specificity of GeneChip were 83.1 and 98.7% for rifampicin resistance, 79.9 and 99.6% for isoniazid resistance, and 74.1 and 99.8% for MDR-TB. CONCLUSIONS: Our experimental results show that the chip method is accurate and reliable; it can be used to detect the type of drug-resistant gene mutation in clinical specimens. Moreover, this study can be used as a reference for future research on TB resistance baselines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12879-018-3131-8) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5964880
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-59648802018-05-24 GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun Zhang, Ming-Jin Ren, Wen-Zhi Sun, Xue-Juan Liu, Yang Liu, Ke-Wei Ji, Zhong-Hao Gao, Wei Yuan, Bao BMC Infect Dis Research Article BACKGROUND: With the widespread use of rifampicin and isoniazid, bacterial resistance has become a growing problem. Additionally, the lack of relevant baseline information for the frequency of drug-resistant tuberculosis (TB) gene mutations is a critical issue, and the incidence of this infection in the city of Changchun has not investigated to date. However, compared with the slow traditional methods of drug susceptibility testing, recently developed detection methods, such as rifampicin and isoniazid resistance-related gene chip techniques, allow for rapid, easy detection and simultaneous testing for mutation frequency and drug resistance. METHODS: In this study, the rifampicin and isoniazid resistance-related gene mutation chip method was employed for an epidemiological investigation. To assess the gene mutation characteristics of drug-resistant TB and evaluate the chip method, we tested 2143 clinical specimens from patients from the infectious diseases hospital of Changchun city from January to December 2016. The drug sensitivity test method was used as the reference standard. RESULTS: The following mutation frequencies of sites in the rifampicin resistance gene rpoB were found: Ser531Leu (52.6%), His526Tyr (12.3%), and Leu511Pro (8.8%). The multidrug-resistance (MDR)-TB mutation frequency was 34.7% for rpoB Ser531Leu and katG Ser315Thr, 26.4% for rpoB Ser531Leu and inhA promoter − 15 (C → T), and 10.7% for rpoB His526Tyr and katG Ser315Thr. In addition, drug susceptibility testing served as a reference standard. In previously treated clinical cases, the sensitivity and specificity of GeneChip were 83.1 and 98.7% for rifampicin resistance, 79.9 and 99.6% for isoniazid resistance, and 74.1 and 99.8% for MDR-TB. CONCLUSIONS: Our experimental results show that the chip method is accurate and reliable; it can be used to detect the type of drug-resistant gene mutation in clinical specimens. Moreover, this study can be used as a reference for future research on TB resistance baselines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12879-018-3131-8) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-22 /pmc/articles/PMC5964880/ /pubmed/29788948 http://dx.doi.org/10.1186/s12879-018-3131-8 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Ming-Jin
Ren, Wen-Zhi
Sun, Xue-Juan
Liu, Yang
Liu, Ke-Wei
Ji, Zhong-Hao
Gao, Wei
Yuan, Bao
GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun
title GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun
title_full GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun
title_fullStr GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun
title_full_unstemmed GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun
title_short GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun
title_sort genechip analysis of resistant mycobacterium tuberculosis with previously treated tuberculosis in changchun
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964880/
https://www.ncbi.nlm.nih.gov/pubmed/29788948
http://dx.doi.org/10.1186/s12879-018-3131-8
work_keys_str_mv AT zhangmingjin genechipanalysisofresistantmycobacteriumtuberculosiswithpreviouslytreatedtuberculosisinchangchun
AT renwenzhi genechipanalysisofresistantmycobacteriumtuberculosiswithpreviouslytreatedtuberculosisinchangchun
AT sunxuejuan genechipanalysisofresistantmycobacteriumtuberculosiswithpreviouslytreatedtuberculosisinchangchun
AT liuyang genechipanalysisofresistantmycobacteriumtuberculosiswithpreviouslytreatedtuberculosisinchangchun
AT liukewei genechipanalysisofresistantmycobacteriumtuberculosiswithpreviouslytreatedtuberculosisinchangchun
AT jizhonghao genechipanalysisofresistantmycobacteriumtuberculosiswithpreviouslytreatedtuberculosisinchangchun
AT gaowei genechipanalysisofresistantmycobacteriumtuberculosiswithpreviouslytreatedtuberculosisinchangchun
AT yuanbao genechipanalysisofresistantmycobacteriumtuberculosiswithpreviouslytreatedtuberculosisinchangchun